CD14+ monocytes, M1-type polarized macrophages, Phenotyping and cell viability (basal)MONO-0011
Monocytes and macrophages are the first defenses against pathogens and take part in the regulation of adaptive immune response. Circulating monocytes enter injured and inflamed peripheral tissues where they differentiate into different types of resident macrophages. As for T helper cells (Th), macrophages are classified into two categories: type 1 macrophages, which are classically activated (M1), and type 2 macrophages that are activated in a more specific way.
M1 macrophages are characterized by a proinflammatory phenotype and an anti-microbial activity. M2 macrophages are involved in angiogenesis, reparation as well as in the matrix remodeling process.
This in vitro assay is performed on CD14+ human monocytes, by using flow cytometry in order to evaluate the capacity of compounds to induce the polarization of monocytes into M1 macrophages (GM-CSF like effect) by phenotype analysis (measurement of the expression level of CD80 and CD163 markers associated with cell viability control).
CD80 and CD163 phenotyping and cell viability (TOPRO-3)
Blood and lymphatic system, cardiovascular system and immunity > Immuno-inflammation assays using blood/immune cells > Monocytes/macrophages
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Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
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Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
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TNF- alpha, IL-6, IL-12p70 release
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|MONO-0007||M1 macrophages, Phenotyping and/or Cytokine release (IFN- gamma + LPS stimulation)||CD80 and CD163 phenotyping and cell viability (TOPRO-3)|
TNF- alpha, IL1 beta, IL-6, IL-10, IL12p70 release
|Flow cytometry (multiplex)|
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|U937-0009||U-937, cytokine release (LPS/PMA stimulation)||*** (must be login)||*** (must be login)|
|THP1-0006||THP-1, caspase 1, enzymatic activity (basal)||Caspase-1 activity||Fluorescence|