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TNFa release
IL-2 release

CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)

CD4TL-0002

  • Context
  • Descriptions
  • Requirement
  • Study and reporting
  • See also
Biological Model

CD4+ T cells

Species

Human

EndPoint

Cytokine release
IL-2, IL-10, IFN- gamma, TNF- alpha release

Method

Flow cytometry (multiplex)

Stimulation Factor

Anti-CD3 (10 µg/ml) + Anti-CD28 (3 µg/ml)

Compound Reference

Cyclosporin A

Physiopathology

Blood and lymphatic system, cardiovascular system and immunity > Immuno-inflammation assays using blood/immune cells > T lymphocytes: anti-inflammatory effects/immunosuppression

Feasibility, study proposal:

Our sales team is here to help you find solutions:
  • Technical feasibility study of your project
  • Development of an assay selection strategy
  • Definition of an experimental scheme
  • Financial assessment and contractual agreements (costs, turnaround time, non-disclosure agreements, etc.)
Please do not hesitate to get in touch with us.

Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)

Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).

Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.

Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Study management: each project requires advice and expertise so each study is managed and monitored by a dedicated study director who will be attentive to all your needs and questions.

Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.

Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.

Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
Reference Title EndPoint Method
Reference Title EndPoint Method
PBMC-0041PBMC, T cell proliferation (Two-Way Mixed Lymphocyte Reaction)Proliferation[3H]-thymidine incorporation
PBMC-0036PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-22 releaseELISA / EIA
PBMC-0009PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
or other Th2 cytokines
TNF- alpha release
ELISA / EIA
THYM-0002Thymocytes, caspase 3 (basal)Active caspase 3Flow cytometry
CD4TL-0009CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
or other Treg cytokines
ELISA / EIA
PBMC-0011PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
or other Treg cytokines
ELISA / EIA
CD4TL-0007CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-17 release
or other Th17 cytokines
ELISA / EIA
PBMC-0010PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-17 release
or other Th17 cytokines
ELISA / EIA
CD4TL-0005CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
IL-4 release
or other Th2 cytokines
TNF- alpha release
ELISA / EIA
PBMC-0008PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IFN- gamma release
IL-2 release
or other Th1 cytokines
TNF- alpha release
ELISA / EIA

Filiale

Bioalternatives, Inc. 250 Park Avenue, 7th Floor, New York, NY, 10177, USA Tel. +1 (212) 364 5115 Mail. sales.us[@]bioalternatives.com

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