Hair follicle, protein expression (basal)FOLL-0003
Hair follicles are located under the skin and are divided into two main parts: one is of epithelial origin and is composed of the matrix (reserve of highly replicative matrix cells essential for hair follicle growth), the shaft, the internal root sheath and the external epithelial root sheath made of keratinocyte filaments; the other part is of mesenchymal origin and comprises the connective tissue sheath made of collagen and proteoglycans and the dermal papilla (reserve of fibroblasts) which is separated from the epithelial compartment by the basement membrane.
The purpose of this assay is to evaluate the stimulating or inhibiting effects of compounds (molecules, active ingredients, extracts, etc.) on hair follicles in culture with regard to the expression and/or localization of relevant proteins (e.g., Ki67, K15, K19, TGK, collagen I, fibronectin, etc.) by immunohistochemistry or immunofluorescence.
Hair follicle (Dissect)
Hair protein markers, e.g. TGK, fibronectin, ...
Skin and cutaneous appendices > Hair > Hair growth and degeneration
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Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)
Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
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|DPF-0002||DPF, proliferation (basal)||Proliferation||BrdU incorporation / Photometry|
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|FOLL-0004||Hair follicle, gene expression (basal)||*** (must be login)||*** (must be login)|