HMVEC + NHDF, differentiation/pseudotube formation (VEGF stimulation, anti-angiogenic agents)ENDOF-0002
Angiogenesis involves the formation of capillaries from preexisting microvessels and therefore contributes to vascular remodeling and maturation. It plays a pivotal function in a variety of normal and pathological conditions such as embryonic development, the menstrual cycle, hair cycle, wound healing, arthritis, psoriasis, proliferative diabetic retinopathy, atherosclerosis, post ischemic vascularization of the myocardium and tumor growth and metastasis. The initiation of the physiopathological angiogenic response, known as the ‘angiogenic switch’, depends on the dynamic balance between exogenous or endogenous stimuli (pro-angiogenic factors) and inhibitors (anti-angiogenic factors) acting in the immediate environment of endothelial cells.
The in vitro assay proposed below consists in a co-culture model of normal human endothelial cells with normal human dermal fibroblasts. After several days of incubation, the treatment of the co-culture with the pro-angiogenic factor VEGF results in the organization of endothelial cells in pseudotubes. Therefore, under VEGF-stimulated condition, this model is suitable to evaluate test compounds capable of inhibiting the formation of this pseudotube network. The pseudotube formation in this model is analyzed by image analysis further to the specific immunolabelling of endothelial cells only using an anti-VWF antibody.
Remark : Under basal conditions (in absence of VEGF), this co-culture model can also be used to screen compounds capable of promoting the development of the pseudotube (assay ENDOF-0001).
HMVEC + Fibroblasts, coculture
Fluorescence / imaging
Skin and cutaneous appendices > Skin vascularization > Angiogenesis
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Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.