M1 macrophages, Phenotyping and/or Cytokine release (IFN- gamma + LPS stimulation)MONO-0007
Monocytes and macrophages are the first defenses against foreign pathogens and take part in the regulation of adaptive immune response. Circulating monocytes enter injured and inflamed peripheral tissues where they differentiate into different types of resident macrophages. As for T helper cells (Th), macrophages are classified into two categories: type 1 macrophages, which are classically activated (M1), and type 2 macrophages that are activated in a more specific way.
M1 macrophages are characterized by a proinflammatory phenotype and an anti-microbial activity. M2 macrophages are involved in angiogenesis, reparation as well as in the matrix remodeling process.
This in vitro assay is performed on M1 macrophages activated by an IFNg+LPS stimulation, in order to evaluate, using flow cytometry, the capacity of compounds to modulate the release of proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-10 and/or IL12p70) and/or to modulate the expression level of CD80 and CD163 markers, respectively described as M1 polarity and M2 polarity markers.
Monocyte-derived macrophages (CD14+), M1 polarized (GM-CSF treatment)
CD80 and CD163 phenotyping and cell viability (TOPRO-3)
Flow cytometry (multiplex)
IFN- gamma (100 ng/ml) + LPS (1 ng/ml)
Blood and lymphatic system, cardiovascular system and immunity > Immuno-inflammation assays using blood/immune cells > Monocytes/macrophages
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Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
|THP1-0007||THP-1, IL-1 beta release (LPS stimulation)||*** (must be login)||*** (must be login)|
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|MONO-0011||CD14+ monocytes, M1-type polarized macrophages, Phenotyping and cell viability (basal)||CD80 and CD163 phenotyping and cell viability (TOPRO-3)||Flow cytometry|
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|U937-0010||U-937, PGE2 release (LPS/PMA stimulation)||*** (must be login)||*** (must be login)|
|U937-0009||U-937, cytokine release (LPS/PMA stimulation)||*** (must be login)||*** (must be login)|
|THP1-0006||THP-1, caspase 1, enzymatic activity (basal)||Caspase-1 activity||Fluorescence|