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ELISA

PBMC, IFN- gamma release (anti-CD28 stimulation)

PBMC-0019

  • Context
  • Descriptions
  • Requirement
  • Study and reporting
  • See also
Biological Model

PBMCs

Species

Human

EndPoint

IFN- gamma release

Method

ELISA / EIA

Stimulation Factor

Anti-CD28 (3 µg/ml)

Physiopathology

Blood and lymphatic system, cardiovascular system and immunity > Immuno-inflammation assays using blood/immune cells > PBMC

Feasibility, study proposal:

Our sales team is here to help you find solutions:
  • Technical feasibility study of your project
  • Development of an assay selection strategy
  • Definition of an experimental scheme
  • Financial assessment and contractual agreements (costs, turnaround time, non-disclosure agreements, etc.)
Please do not hesitate to get in touch with us.

Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)

Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).

Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.

Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Study management: each project requires advice and expertise so each study is managed and monitored by a dedicated study director who will be attentive to all your needs and questions.

Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.

Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.

Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
Reference Title EndPoint Method
Reference Title EndPoint Method
PBMC-0042PBMC, M2-type macrophage induction in a MLR (Mix Lymphocyte Reaction) culture (basal)*** (must be login)*** (must be login)
PBMC_Lb-0001PBMC, PGE2 release (LPS stimulation)*** (must be login)*** (must be login)
PBMC-0041PBMC, T cell proliferation (Two-Way Mixed Lymphocyte Reaction)Proliferation[3H]-thymidine incorporation
PBMC-0038PBMC, inflammation gene expression (LPS stimulation)qPCRarray (16 genes)qPCRarray (standard or customized)
PBMC-0037PBMC, PI3K/AKT/mTOR or MAPkinases activation (Basal)AKT phosphorylation
ERK1/2 phosphorylation
ELISA / EIA
PBMC-0036PBMC, anti-CD3/anti-CD28-stimulated T cells, Th22 cytokine release (anti-CD3/anti-CD28 stimulation)IL-22 releaseELISA / EIA
PBMC-0009PBMC, anti-CD3/anti-CD28-stimulated T cells, Th2 cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
IL-13 release
IL-5 release
or other Th2 cytokines
TNF- alpha release
ELISA / EIA
BMC-0004Microglia, cytokine release (fractalkine stimulation)IL-1 beta release
IL-6 release
TNF- alpha release
ELISA / EIA
PBMC-0013PBMC, LPS-stimulated monocytes, cytokine release (LPS stimulation)Cytokine release
IFN- gamma release
IL-1 beta release
IL-6, IL-8, IL-12/IL-23p40, TNF- alpha release
MIP-1alpha release
Flow cytometry (multiplex)
PBMC-0011PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)IL-10 release
or other Treg cytokines
ELISA / EIA

Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

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Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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