Preadipocytes, differentiation, PPAR- gamma expression (differentiation mix stimulation)PREAD-0016
The PPAR-γ nuclear receptor (Peroxisome proliferator-activated receptor gamma) can be found among various types of cells and is involved in regulating the expression of genes associated with cell growth and cell differentiation.
The PPAR-γ receptor is principally involved in adipocyte differentiation (adipogenesis) and coordinates the expression of several hundreds of genes that provide a phenotype of mature adipocytes. Adipocyte differentiation is regulated by a cascade of transcriptional events in which the PPAR-γ receptor and other members of C/EBP family play a significant role.
This in vitro assay is performed, using Western blot, on a model of human preadipocytes under differentiation conditions, in order to evaluate the capacity of compounds to inhibit the PPAR-γ receptor expression.
PreAd (human preadipocyte)
PPAR- gamma expression
Skin and cutaneous appendices > Adipocyte, hypodermis > Adipocyte differentiation
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Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
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Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
|PREAD-0011||Preadipocytes, differentiation, lipid droplet formation||*** (must be login)||*** (must be login)|
|HMSC-0001||HMSC differentiation in adipocytes||*** (must be login)||*** (must be login)|
|3T3L1-0010||3T3-L1, differentiation, lipid content/analysis||*** (must be login)||*** (must be login)|
|PREAD-0007||Preadipocytes, differentiation, lipid content||Lipid labeling||Fluorescence / imaging|
|PREAD-0009||Preadipocytes, adipogenic differentiation markers (differentiation mix stimulation)||mQPA-ADIPO-16|
mRNA: e.g. LPL, FABP4, PPARG, COL1A1...
|qPCRarray (standard or customized)|