RHE, [14C]-caffein diffusion (basal)EPIBA-0019
The epidermis provides a physical and permeability barrier which is continuously regenerated by the epidermal keratinocyte differentiation process. Multiple components can impact the barrier function quality including protein structure (claudin, desmoglein, filaggrin, etc.), lipids (ceramides) and proteases. When properly differentiated, the epidermis prevents water loss and provides a barrier to epidermal invasion of pollutants, toxins, allergens and bacteria.
The purpose of this assay is to evaluate, via radiodetection, the capacity of compounds (active ingredients or formulations) to reinforce the human reconstructed epidermis barrier function by analyzing caffeine diffusion.
Reconstructed Epidermis, Bioalternatives RHE
[14C]-caffein diffusion (long protocol; kinetic)
Skin and cutaneous appendices > Epidermis/keratinocyte physiology > Differentiation, barrier function and hydration > Barrier function
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- Technical feasibility study of your project
- Development of an assay selection strategy
- Definition of an experimental scheme
- Financial assessment and contractual agreements (costs, turnaround time, non-disclosure agreements, etc.)
Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)
Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
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|qPCRarray (standard or customized)|