RHE, mRNA for differentiation markers (basal)EPIBA-0022
The epidermis provides a physical and permeability barrier which is continuously regenerated by the epidermal keratinocyte differentiation process. During this process, keratinocytes migrate from the basal lamina to the outermost layer of the epidermis and form the stratum corneum. Multiple components can impact the barrier function quality, including protein structure (claudin, desmoglein, filaggrin, etc.), lipids (ceramides) and proteases. When properly functioning, the epidermis prevents water loss and provides a barrier to epidermal invasion of allergens and bacteria.
The purpose of this in vitro assay, performed on reconstructed human epidermis (RHE), is to evaluate the effects of compounds on specific gene expression of epidermal keratinocytes.
Transcriptomic analysis is performed from a selection of 16 or 64 genes, using RT-qPCR where genes of each list are grouped according to biological function (lipid synthesis pathways, epidermal differentiation process, dermal-epidermal junction, intracellular interaction, etc.).
Reconstructed Epidermis, Bioalternatives RHE
qPCRarray (standard or customized)
Retinoic acid (all trans)
Skin and cutaneous appendices > Epidermis/keratinocyte physiology > Differentiation, barrier function and hydration > Epidermal differentiation markers
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- Technical feasibility study of your project
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- Definition of an experimental scheme
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Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)
Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.