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Ceramides and cerebrosides synthesis
Total lipids - TLC

RHE, phospholipid & ceramide neosynthesis (basal)

EPIBA-0024

  • Context
  • Descriptions
  • Requirement
  • Study and reporting
  • See also
Epidermal lipids are polar lipids located above the granular layer. They form large lamellae which provide intercorneocytory cement. Epidermal lipids thus play a major role in the skin barrier function and in the maintaining of skin hydration.   The purpose of this assay is to evaluate the effects of compounds (active ingredients or formulations) on lipid neosynthesis in reconstructed human epidermis by measuring  [14C]-acetate incorporation into various lipid species (total lipids, ceramides, cerebrosides, cholesterol sulfate, etc.).  
Biological Model

Reconstructed Epidermis, Bioalternatives RHE

Species

Human

EndPoint

Phospholipid and ceramide neosynthesis

Method

[14C]-acetate incorporation + TLC + image analysis (semi-quantitative method)

Stimulation Factor

Basal

Physiopathology

Skin and cutaneous appendices > Epidermis/keratinocyte physiology > Differentiation, barrier function and hydration > Epidermal lipids

Feasibility, study proposal:

Our sales team is here to help you find solutions:
  • Technical feasibility study of your project
  • Development of an assay selection strategy
  • Definition of an experimental scheme
  • Financial assessment and contractual agreements (costs, turnaround time, non-disclosure agreements, etc.)
Please do not hesitate to get in touch with us.

Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)

Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).

Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.

Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Study management: each project requires advice and expertise so each study is managed and monitored by a dedicated study director who will be attentive to all your needs and questions.

Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.

Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.

Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
Reference Title EndPoint Method
Reference Title EndPoint Method
SPL_Lb-0017Biological sample analysis - Analysis of sebum composition (neutral lipids)*** (must be login)*** (must be login)
SKIN_Lb-0007Skin maintained ex vivo, analysis of mineral elements (NMF) (basal)*** (must be login)*** (must be login)
SKIN_Lb-0006Skin maintained ex vivo, analysis of filaggrin catabolites: serine, UCA and PCA (NMF) (basal)*** (must be login)*** (must be login)
SKIN_Lb-0005Skin maintained ex vivo, analysis of filaggrin catabolites: serine, UCA and PCA (NMF) (cytokine stimulation)*** (must be login)*** (must be login)
SKIN_Lb-0004Skin maintained ex vivo, analysis of ceramides (polar lipids) (cytokine stimulation)*** (must be login)*** (must be login)
RHE_Lb-0011RHE, analysis of mineral elements (NMF) (basal)*** (must be login)*** (must be login)
RHE_Lb-0010RHE, analysis of filaggrin catabolites: serine, UCA and PCA (NMF) (basal)*** (must be login)*** (must be login)
RHE_Lb-0009RHE, analysis of filaggrin catabolites: serine, UCA and PCA (NMF) (cytokine stimulation)*** (must be login)*** (must be login)
SPL_Lb-0006Biological sample analysis - Analysis of urea and lactate (NMF)*** (must be login)*** (must be login)
SKIN_Lb-0003Skin maintained ex vivo, analysis of ceramides (polar lipids) (basal)*** (must be login)*** (must be login)

Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

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Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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FTSK, mRNA for differentiation markers (basal) qPCR Hyaluronic acid_SKIN_IF Skin maintained ex vivo, hyaluronic acid expression (basal) Scroll to top

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