Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm

According to the International Bureau of Weights and Measurements, metrology, the study of measurement, is becoming an essential aspect of all industries today in creating value.

Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo

Loss of melanocytes is the pathological hallmark of vitiligo.

How In Silico Screening Can Guide Product Development in Cosmetics

What's the role of in silico screening in cosmetic product development? Read on to learn more about the benefits of in silico screening and how it works.
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The Relationship Between Microbiology Testing and the Skin Microbiota

Microbiology testing is essential for substantiating microbiome-friendly and anti-microbial claims. Read on to find out how cosmetic microbiology testing is connected to the skin microbiome.

Using Reconstructed Human Epidermis model in Skin Microbiome Research

Skin microbiome research is developing rapidly. Are you up to date? Find out why 3D skin models are an important development in skin microbiome research.

Canities and hair pigmentation

Canities (hair greying) has a wide-ranging social and cosmetic impact. Bioalternatives is focusing on its physiopathology, underlying causes and treatments.
in vitro and ex vivo assays: skin barrier and hydration

Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation

In this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains...
in vitro and ex vivo assays: skin ageing

How are In Vitro Testing Methods Used for Cosmetics?

With the growing distaste and outright bans of animal testing of cosmetic products, more and more cosmetics companies are turning to in vitro testing. Let’s take a closer look at the nature of in vitro testing and the opportunities it offers for cosmetics brands and manufacturers.

Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.

Androgens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.

Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.

Acne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.

Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.

We analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.

Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.

Androgens are key regulators of sebaceous function... A link between mTOR and androgen signaling has previously been well as a link between these processes and lipid synthesis...Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
in vitro and ex vivo assays: skin protection and immune defense system

Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene

In this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.