https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2018/09/mw_Pseudomonas.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2018-09-11 09:32:112018-09-11 10:26:11Pseudomonas aeruginosa flagellum is critical for invasion, cutaneous persistence and induction of inflammatory response of skin epidermis
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2018/03/mw_New-biological-activities-of-Lythrum-salicaria-L..jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2018-03-19 12:08:042018-03-20 14:41:15New Biological Activities of Lythrum salicaria L.
The perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/SEPPIC-Image-à-la-une.jpg 640 1140 Linhda Coulevard https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Linhda Coulevard2017-11-29 10:57:192017-11-29 11:44:39Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
Sebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Image-à-la-une.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-11-29 09:30:252018-06-01 14:10:54Acne vulgaris: physiopathology and cell mechanisms
Acne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization of the pilosebaceous follicle by certain bacteria (P. acnes). Bioalternatives has developed a panel of innovative assays to discover and evaluate compounds for the treatment of acne.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Sébocytes-image-à-la-une.jpg 368 701 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-11-28 09:24:342018-06-01 14:11:31Physiology and functions of the sebaceous gland
The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/04/structure-cheveu-Image-à-la-une.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-06-07 10:27:462017-10-27 14:36:00Hair: Follicle, Associated Structures and Growth
Hair follicles are skin adnexa which are able to regenerate in a complete, autonomous, cyclic and asynchronous manner. The histological analysis of the pilosebaceous unit reveals a three-element organization: the epithelial compartment, the mesenchymal compartment and the sebaceous gland.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/03/YR-Image-à-la-une.jpg 368 655 Linhda Coulevard https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Linhda Coulevard2017-03-24 12:03:322017-12-14 10:54:29New biological effects of Aphloïol
In the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/03/Silab-Image-à-la-une.jpg 350 623 Linhda Coulevard https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Linhda Coulevard2017-03-21 18:10:582017-03-23 14:46:05in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
Development of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/12/Epiderme-reconstruit-passage-2-figure-1A-1.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2016-12-21 16:21:562017-01-20 09:45:20Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
The development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_androgens-induce-sebaceous-differentiation-in-sebocyte.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2015-04-09 15:47:492016-12-29 14:04:54Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor
In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662  constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Cystinosin-is-a-melanosomal-protein.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2012-05-14 09:05:322016-12-05 12:01:02Cystinosin is a melanosomal protein that regulates melanin synthesis
In vitro studies showed that cystinosin was located at melanosomes. CTNS silencing led to a 75% reduction of melanin synthesis that was caused by a degradation of tyrosinase by lysosomal proteases. Our results objectify the pigmentation defect in patients with cystinosis. We also identify the role of CTNS in melanogenesis and add a new gene to the list of the genes involved in the control of skin and hair pigmentation.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Immortalized-sebocytes.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2012-01-24 10:17:152016-12-29 14:05:00Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium
SEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Effect-of-C-xyloside.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2011-03-24 10:58:512016-12-05 12:02:11Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
These data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Lipolytic-effect-of-a-polyphenolic-citrus-dry-extract.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2008-07-09 14:18:052016-12-05 12:06:34Lipolytic effect of a polyphenolic citrus dry extract of red orange, grapefruit, orange (Sinetrol) in human body fat adipocytes. Mechanism of action by inhibition of cAMP-Phosphodiesterase (PDE)
The present study investigated the lipolytic (break of fat stored) effect of a citrus-based polyphenolic dietary supplement (SINETROL) at human adipocytes (ex vivo), body fat (clinical) and biochemical levels (inhibition of phosphodiesterase).
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Tensin1.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2008-06-04 16:13:102016-12-05 12:06:53Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
tensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_UV-induced-effects.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2005-05-30 10:47:442016-12-05 12:10:29Alternative (non-animal) methods for cosmetic testing: current status and future prospects - UV-induced effects
Cosmetic ingredients and mixtures of ingredients absorbing UV light (in particular UV filter chemicals used, for example, to ensure the light stability of cosmetics or used in sun protection products) should be tested for acute phototoxic and photogenotoxic potential.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Acute-toxicity.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2005-05-30 10:22:572016-12-05 12:10:47Alternative (non-animal) methods for cosmetic testing: current status and future prospects - Acute toxicity
From the regulatory point of view, the main objective of acute toxicity testing is basically to classify chemicals according to their intrinsic toxicity
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Comparison-of-gene-expression-profiles.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2002-02-10 11:25:232016-12-05 12:12:01Comparison of gene expression profiles in human keratinocyte mono-layer cultures, reconstituted epidermis and normal human skin; transcriptional effects of retinoid treatments in reconstituted human epidermis
In order to validate a model for predictive screening of dermatological drugs, we used a customized cDNA macro-array system containing 475 skin-related genes to analyze the gene expression patterns in human keratinocytes from different origins.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Development-of-a-highly-in-vitro-phototoxicity-assay.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2000-12-15 13:01:422016-12-05 12:12:26Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic reconstructed human epidermis
The reconstituted human epidermis model SkinEthic was used to evaluate the phototoxicity of topically applied chemicals.