https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Acné-généralité-image-à-la-une-_-EN.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-11-29 09:31:592018-06-01 14:11:41Acne vulgaris: Origins, symptoms and current treatments
Acne vulgaris is a chronic inflammatory disease of the skin which affects most adolescents and is the cause of over half of all dermatology consultations. This pathology, which is characterized by a hypersecretion of sebum, can be the cause of physical complications and may also lead to psychological distress.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Image-à-la-une.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-11-29 09:30:252018-06-01 14:10:54Acne vulgaris: physiopathology and cell mechanisms
Acne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization of the pilosebaceous follicle by certain bacteria (P. acnes). Bioalternatives has developed a panel of innovative assays to discover and evaluate compounds for the treatment of acne.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Sébocytes-image-à-la-une.jpg 368 701 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2017-11-28 09:24:342018-06-01 14:11:31Physiology and functions of the sebaceous gland
The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/08/IL-17A.png 368 655 Linhda Coulevard https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Linhda Coulevard2017-07-14 09:37:162017-08-30 13:55:54Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis
Acute-serum Amyloid A (A-SAA), one of the major acute-phase proteins, is mainly produced in the liver but extra-hepatic synthesis involving the skin has been reported. Its expression is regulated by the transcription factors NF-κB, C/EBPβ, STAT3 activated by proinflammatory cytokines.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/11/mw_SMA.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2016-11-12 15:36:082016-12-09 09:44:49Wound healing, granulation phase and maturation phase
The third phase of wound healing, consisting in the replacement of the provisional fibrin matrix with granulation tissue once the wound has been debrided, includes several sub-phases: re-epithelialization, fibroplasia, collagen deposition and angiogenesis.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/11/mw_blood-cells-cicatrisation-inflammation-AdobeStock_117910248.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2016-11-10 15:40:392016-12-06 11:10:52Wound healing, hemostasis phase and inflammatory phase
The inflammatory phase is characterized by the sequential infiltration of polymorphonuclear neutrophils (PMNs), monocytes/macrophages and lymphocytes. IL-8 facilitates PMNs migration from surrounding blood vessels.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/pf_Cicatrisation-Adobe.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2016-11-09 15:45:552016-12-09 09:44:18Wound healing, overview
Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells , stem cells , hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves and mechanical forces.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_imq-induced-skin-inflammation.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2015-07-24 17:30:202016-12-05 10:48:40Imiquimod-induced skin inflammation in mice is dependent on IL-1R1 and MyD88 signaling but independent of the NLRP3 inflammasome
The pathogenesis of inflammatory skin diseases such as psoriasis involves the release of numerous proinflammatory cytokines, including members of the IL-1 family. Here we report overexpression of IL-1α, IL-1β, and IL-1 receptor antagonist mRNA, associated to expression of IL-23p19, IL-17A, and IL-22 in skin cells
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_androgens-induce-sebaceous-differentiation-in-sebocyte.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2015-04-09 15:47:492016-12-29 14:04:54Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor
In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662  constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_study-of-proliferation-and-3D-epidermal-reconstruction-from-foreskin.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2014-09-16 16:18:492016-12-05 11:57:38Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children
Our studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested the hypothesis that in vitro studies and RHE reconstructive capacities of cells from different body sites can be helpful to select an optimal site for keratinocyte isolation before considering graft protocols for girls.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_epidermal-healing-in-burns.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2014-09-07 16:13:052017-05-12 11:47:33Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective
In the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_inhibition-of-keratinocyte-differentiation.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2014-07-10 16:22:302016-12-05 11:58:41Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1α, TNFα and oncostatin M
This study highlights the precise role of cytokines in the skin inflammatory response (psoriasis). IL-22 and OSM more specifically drive epidermal hyperplasia and differentiation loss while IL-1α, IL-17A and TNFα were more involved in the activation of innate immunity.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2013/04/mw_Contribution-of-IL-22.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2013-04-11 15:14:312016-12-05 11:59:28Contribution of IL22 to experimental skin inflammation
Focused on in vitro human models, we present the mechanisms of action of IL22 as well as its involvement in structure, metabolism, differentiation, chemotaxis, antibacterial activity, innate immunity, and tissue remodeling of epidermis.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_Depositphotos_pied-bébé.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2013-04-10 12:48:122018-06-01 14:12:44Atopic dermatitis: physio-pathology and clinical signs
Atopic dermatitis is the most common dermatosis affecting children: 65% of the patients are less than a year old and 85% are below 5 years. The prevalence of this pathology is constantly on the rise and currently affects 10 to 25% of the population.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_foreskin-isolated-keratinocytes-provide.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2013-03-14 16:32:272016-12-05 12:00:14Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts
We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. in vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_keratinocytes-under-fire.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2012-10-05 16:11:462016-12-05 12:00:40Keratinocytes under fire of proinflammatory cytokines: Bonafide innate immune cells involved in the physiopathology of chronic atopic dermatitis and psoriasis
Specific cytokine environment deregulation plays a central role on skin morphology and innate immunity, moving towards specific pathologies and opening the way to new therapeutic strategies.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_Inflammatory-loop-ter-1.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2012-03-26 12:56:432018-06-01 14:12:13Atopic dermatitis - initiation and chemokines activation step
The skin lesions facilitate the passage of an antigen that the subject has previously been sensitized to.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/03/mw_Eczema-sub-aigux20.jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Julie2012-03-25 13:01:112018-06-01 14:12:07Atopic dermatitis - immune response and skin lesions
TH2/TH22 immune response and other agents of atopic dermatitis After polarization, the Th2 and Th22 TL migrate to the lesion area, where they release type Th2 (IL-4, IL-5, IL-13, IL-31, TNF-α) and Th22 (IL-22) cytokines respectively. These Th2/Th22 cytokines have different functions in the immunity response.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Immortalized-sebocytes.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2012-01-24 10:17:152016-12-29 14:05:00Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium
SEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2016/05/mw_Foreskin-in-children.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Guillaume2010-12-10 15:11:492016-12-05 12:02:52Quantitative and qualitative study in keratinocytes from foreskin in children: Perspective application in paediatric burns
Keratinocytes from foreskin have a high capacity for division. A potential source of cells to provide coverage in paediatric burns.