Bioalternatives has many in vitro or ex vivo models at your disposal:
- normal human epidermal keratinocytes (NHEK)
- reconstructed human epidermis (RHE)
- sebocytes (SEBO662AR)
- full thickness skin (FTSK)
- skin explants (ex vivo)
on which we can evaluate case by case the hydrating effect of active compounds or formulations by measuring:
- the reinforcement of the barrier function
- the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
- lipid synthesis (acid mantle):
- sebaceous lipids
- epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
- the expression or synthesis of epidermal extracellular matrix components:
- glycosaminoglycans and hyaluronic acid
- proteoglycans and ECM receptors
- proteases (e.g. MMPs)
- the expression of markers of epidermal cohesion and intercellular cell junctions:
- occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
- dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
- gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
Here are a few examples among all standard assays proposed by Bioalternatives in the field of hydration and reinforcement of the barrier function:
New Biological Activities of Lythrum salicaria L.
/in Skin barrier and hydration /by JulieThe perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
Physiology and functions of the sebaceous gland
/in Acne, Sebaceous gland regulation, Skin barrier and hydration /by JulieThe sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.
New biological effects of Aphloïol
/in Cosmetics, Skin barrier and hydration /by Linhda CoulevardIn the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.