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Bioalternatives
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  • Cosmetics
    • in vitro efficacy assays
      • Hydration and skin barrier
      • Epidermal regeneration
      • Skin firmness & cohesion
      • Skin ageing
      • Skin protection & defense
      • Skin pigmentation
      • Oily skin and hyperseborrhea
      • Skin microbiome
      • Slimming and adipocyte metabolism
      • Skin microcirculation and vascularization
      • Hair growth and alopecia
    • Bioanalysis and bioengineering
      • Cell and tissue engineering
      • Gene expression and transcriptomic analysis
      • Histology, Immunohistochemistry, Immunofluorescence
      • Development and validation of assays and protocols
  • Dermatology
    • Preclinical research
      • Acne
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      • Atopic dermatitis
      • Wound healing and skin regeneration
      • Psoriasis
      • Melanoma
      • Vitiligo (coming soon)
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      • Development and validation of assays and protocols
      • Gene expression and transcriptomic analysis
      • Histology, Immunohistochemistry, Immunofluorescence
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      • Health animal and veterinary medicine
      • Neurobiology
    • Bioanalysis and bioengineering
      • Cell and tissue engineering
      • Development and validation of assays and protocols
      • Gene expression and transcriptomic analysis
      • Histology, Immunohistochemistry, Immunofluorescence
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HYDRATION AND SKIN BARRIER

Skin hydration partly determines the aspect of the skin (dry, rough and oily skin, etc.). Through its barrier function, skin is our shield from external stress factors and prevents the evaporation of such essential elements as water, ions and amino acids.

Skin hydration is mostly linked to the skin barrier function. The role of a moisturizing skin care product is to act on skin surface to physically limit water loss (occlusive effect) or to act at the cellular level via different biological action models:

  • reinforcement of the barrier function and of epidermal differentiation
  • restoration of skin lipids
  • stimulation of hygroscopic endogenous factors (water retention)
  • improvement of cellular exchanges in solutes and in water

Hydration is a multifactorial process that cannot be understood through a single assay or model. It is therefore more conclusive to use a panel of complementary efficacy assays in order to study and demonstrate the hydrating properties of an active compound or cosmetic product.

HYDRATION AND REINFORCEMENT OF THE SKIN BARRIER FUNCTION: MODELS AND ASSAYS

Bioalternatives has many in vitro or ex vivo models at your disposal:

  • normal human epidermal keratinocytes (NHEK)
  • reconstructed human epidermis (RHE)
  • sebocytes (SEBO662AR)
  • full thickness skin (FTSK)
  • skin explants (ex vivo)

on which we can evaluate case by case the hydrating effect of active compounds or formulations by measuring:

  • the reinforcement of the barrier function
  • the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
  • lipid synthesis (acid mantle):
    • sebaceous lipids
    • epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
  • the expression or synthesis of epidermal extracellular matrix components:
    • glycosaminoglycans and hyaluronic acid
    • proteoglycans and ECM receptors
    • proteases (e.g. MMPs)
  • the expression of markers of epidermal cohesion and intercellular cell junctions:
    • occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
    • dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
    • gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
Full thickness skin (loricrin in green)
Normal human epidermal keratinocytes (filaggrin)
Skin explants (lipids)
Skin explants (CD44)
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Here are a few examples among all standard assays proposed by Bioalternatives in the field of hydration and reinforcement of the barrier function:

  • NHEK, desmosomal, tight & other cell-cell adhesion proteins (basal)

    1.00€
    Add to cart Show Details
  • NHEK, epidermal barrier markers (mRNA) (basal)

    1.00€
    Add to cart Show Details
  • NHEK, hyaluronic acid synthesis / release (basal)

    1.00€
    Add to cart Show Details
  • RHE, phospholipid & ceramide neosynthesis (basal)

    1.00€
    Add to cart Show Details
  • RHE, [14C]-caffein diffusion (basal)

    1.00€
    Add to cart Show Details
  • Skin maintained ex vivo, protein marker (proteoglycans, ECM receptors…) expression (basal)

    1.00€
    Add to cart Show Details
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Discover all our standard assays related to skin hydration

Direct access to catalog

Hydration and skin barrier: posts and publications

New Biological Activities of Lythrum salicaria L.

19 March 2018/in Skin barrier and hydration /by Julie

The perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.

Read more
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2018/03/mw_New-biological-activities-of-Lythrum-salicaria-L..jpg 368 655 BA_Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png BA_Julie2018-03-19 12:08:042018-03-20 14:41:15New Biological Activities of Lythrum salicaria L.

Physiology and functions of the sebaceous gland

28 November 2017/in Acne, Sebaceous gland regulation, Skin barrier and hydration /by Julie

The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.

Read more
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/11/Sébocytes-image-à-la-une.jpg 368 701 BA_Julie https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png BA_Julie2017-11-28 09:24:342018-02-13 10:44:49Physiology and functions of the sebaceous gland
Aphloïol barrier function

New biological effects of Aphloïol

24 March 2017/in Cosmetics, Skin barrier and hydration /by Linhda Coulevard

In the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.

Read more
https://cdn-bneoicczbswi.netdna-ssl.com/wp-content/uploads/2017/03/YR-Image-à-la-une.jpg 368 655 Linhda Coulevard https://www.bioalternatives.com/wp-content/uploads/2017/03/logo-Bioalternatives-1.png Linhda Coulevard2017-03-24 12:03:322017-12-14 10:54:29New biological effects of Aphloïol

Cosmetics

  • Epidermal regeneration
  • Hair growth and alopecia
  • Hydration and skin barrier
  • Oily skin and hyperseborrhea
  • Skin ageing
  • Skin firmness & cohesion
  • Skin microbiome
  • Skin microcirculation and vascularization
  • Skin pigmentation
  • Skin protection & defense
  • Slimming and adipocyte metabolism

Cosmetics

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Health animal and veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)

Online account and catalog access

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Posts and publications

  • New Biological Activities of Lythrum salicaria L.
  • Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
  • Acne vulgaris: Origins, symptoms and current treatments
  • Acne vulgaris: physiopathology and cell mechanisms
  • Physiology and functions of the sebaceous gland

Headquarters

Bioalternatives SAS
1 bis rue des plantes BP 50011
86160 Gençay, France
Tel. +33 (0)5 49 36 11 37
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