Bioalternatives has many in vitro or ex vivo models at your disposal:
- normal human epidermal keratinocytes (NHEK)
- reconstructed human epidermis (RHE)
- sebocytes (SEBO662AR)
- full thickness skin (FTSK)
- skin explants (ex vivo)
on which we can evaluate case by case the hydrating effect of active compounds or formulations by measuring:
- the reinforcement of the barrier function
- the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
- lipid synthesis (acid mantle):
- sebaceous lipids
- epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
- the expression or synthesis of epidermal extracellular matrix components:
- glycosaminoglycans and hyaluronic acid
- proteoglycans and ECM receptors
- proteases (e.g. MMPs)
- the expression of markers of epidermal cohesion and intercellular cell junctions:
- occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
- dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
- gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
Here are a few examples among all standard assays proposed by Bioalternatives in the field of hydration and reinforcement of the barrier function:
New Biological Activities of Lythrum salicaria L.
/in Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration /by JulieThe perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
La glande sébacée : Physiologie et fonctions
/in Acné, Barrière cutanée et hydratation, Peau grasse, hyperséborrhée et séborégulation /by GuillaumeLa glande sébacée est un organe localisé au niveau du derme. Elle est responsable de la synthèse et de la sécrétion du sébum qui entre dans la composition du film hydrolipidique. Le rôle du sébum est de protéger la peau des agressions extérieures et de la déshydratation. Le sébum permet également d’entretenir la souplesse de la peau et des cheveux.
New biological effects of Aphloïol
/in Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration /by GuillaumeIn the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.