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Bioalternatives
  • Bioalternatives
    • About us
    • Our team
    • Publications
    • Careers
  • Cosmetics
    • Hydration and skin barrier
    • Epidermal regeneration
    • Skin firmness & cohesion
    • Skin ageing
    • Skin protection & defense
    • Skin pigmentation
    • Oily skin and hyperseborrhea
    • Skin microbiota
    • Slimming and adipocyte metabolism
    • Skin microcirculation and vascularization
    • Hair growth, alopecia & canities
  • Dermatology
    • Acne
    • Skin microbiota
    • Alopecia, hair growth & canities
    • Atopic dermatitis
    • Wound healing and skin regeneration
    • Psoriasis
    • Melanoma
    • Vitiligo (coming soon)
  • Pharmacology
    • Immuno-Inflammation
    • Veterinary medicine
    • Neurobiology
  • Bioanalysis and bioengineering
    • Analytical and bioanalytical services
      • Bioanalysis of non-invasive clinical samples
    • Cell and tissue engineering
    • Chemoinformatics, in silico virtual screening & phytochemical profiling
      • in silico screening – SELNERGY
      • Phytochemical analysis & metabolic profiling – GAINS
    • Gene expression and transcriptomic analysis
      • RNA extraction and quality control
      • Rt-qPCR & PCRarrays
      • Microarrays
      • miRNA
      • In situ hybridization
      • Bioinformatics
    • Histology services
      • Slide preparation
      • Histological staining
      • Immunolabeling
      • Electron microscopy TEM/SEM
      • Slide scanning
    • Development and validation of assays and protocols
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HYDRATION AND SKIN BARRIER

Skin hydration partly determines the aspect of the skin (dry, rough and oily skin, etc.). Through its barrier function, skin is our shield from external stress factors and prevents the evaporation of such essential elements as water, ions and amino acids.

Skin hydration is mostly linked to the skin barrier function. The role of a moisturizing skin care product is to act on skin surface to physically limit water loss (occlusive effect) or to act at the cellular level via different biological action models:

  • reinforcement of the barrier function and of epidermal differentiation
  • restoration of skin lipids
  • stimulation of hygroscopic endogenous factors (water retention)
  • improvement of cellular exchanges in solutes and in water

skin proteic structure, physico biochemical barrier

Hydration is a multifactorial process that cannot be understood through a single assay or model. It is therefore more conclusive to use a panel of complementary efficacy assays in order to study and demonstrate the hydrating properties of an active compound or cosmetic product.

skin barrier and hydration

CONTACT OUR TEAM TO WATCH THE REPLAY

HYDRATION AND REINFORCEMENT OF THE SKIN BARRIER FUNCTION: MODELS AND ASSAYS

Models

Bioalternatives has many in vitro or ex vivo models at your disposal:

  • normal human epidermal keratinocytes (NHEK)
  • normal reconstructed human epidermis (RHE) & Reconstructed human epidermis in default
  • sebocytes (SEBO662AR)
  • full thickness skin (FTSK)
  • skin explants (ex vivo)
Filaggrin labeling in normal human keratinocytes
Labeling of SEBO662AR cells treated by DHT (Bodipy in green, EMA in red and nuclei in blue)
Kallikrein-5 labeling in RHE
Loricrin labeling in full thickness skin
Caspase-14 labeling in Skin explant
EMA immunolabeling in 3D sebocyte model
Evaluation of skin barrier and hydration

We can evaluate case by case the hydrating effect of active compounds or formulations by measuring:

  • the reinforcement of the barrier function
  • the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
  • lipid synthesis (acid mantle):
    • sebaceous lipids
    • epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
  • the expression or synthesis of epidermal extracellular matrix components:
    • glycosaminoglycans and hyaluronic acid
    • proteoglycans and ECM receptors
    • proteases (e.g. MMPs)
  • the expression of markers of epidermal cohesion and intercellular cell junctions:
    • occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
    • dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
    • gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
skin physico biochemical barrier evaluation

Here are a few examples among all standard assays proposed by Bioalternatives in the field of hydration and reinforcement of the barrier function:

  • Claudin-1-expression#Ref

    NHEK, desmosomal, tight & other cell-cell adhesion proteins (basal)

    1.00€
    Add to cart Show Details
  • Calcium-induced gene expression

    NHEK, epidermal barrier markers (mRNA) (basal)

    1.00€
    Add to cart Show Details
  • Hyaluronic acid synthesis

    NHEK, hyaluronic acid synthesis / release (basal)

    1.00€
    Add to cart Show Details
  • Ceramides and cerebrosides synthesis

    RHE, phospholipid & ceramide neosynthesis (basal)

    1.00€
    Add to cart Show Details
  • Caffein diffusion

    RHE, transepidermal permeation and barrier function (basal)

    1.00€
    Add to cart Show Details
  • E920_Derme novolink_Fibuline 3

    Skin maintained ex vivo, protein marker (proteoglycans, ECM receptors…) expression (basal)

    1.00€
    Add to cart Show Details
PreviousNext

HYDRATION AND REINFORCEMENT OF THE SKIN BARRIER FUNCTION: CLINICAL BIOANALYSIS

Analysis of lipids involved in the barrier function

Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.

The epidermal lipids involved in the barrier function of the epidermis (ceramides, fatty acids and cholesterol) are removed using the SW Kit.

The analysis of these lipids makes it possible to evaluate the quality of the intercorneocyte cement involved in the barrier function of the epidermis and in the prevention of transepidermal water loss (TEWL).

These evaluations help support your claims about the efficacy of biomimetic products, barrier products, protective products, moisturizers, etc.

Analysis of the components of the Natural Moisturizing Factor (NMF)

The amino acids and minerals present on the surface of the skin are collected using the SW Kit:

  • PCA / UCA (cis/trans) – (catabolites of filaggrin)
  • Amino acids
  • Urea, lactates
  • Mineral elements: Ca, K, Na, Mg, Zn, etc.

The analysis of these compounds makes it possible to evaluate the impact of the NMF component on skin hydration.

These analysis help support your claims about the efficacy of hygroscopic products, barrier products, protective products, moisturizers, etc.

Screening des céramides

Ceramide screening – LC/MS

–

Cornéocytes endommagés et sains - SEMX500

Damaged and healthy corneocytes – SEMX500

–

Analyse des PCA - LC/UV

PCA analysis – LC/UV

Find out about all our standard assays - Direct access to catalog
A project, an idea or a question? Contact us & let’s talk about it!

Hydration and skin barrier: posts and publications

New Biological Activities of Lythrum salicaria L.

Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration

The perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.

Read more
19 March 2018/by Julie
https://www.bioalternatives.com/wp-content/uploads/2018/03/mw_New-biological-activities-of-Lythrum-salicaria-L..jpg 368 655 Julie https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Julie2018-03-19 12:08:042019-09-13 21:33:51New Biological Activities of Lythrum salicaria L.
glande sébacée

La glande sébacée : Physiologie et fonctions

Acné, Barrière cutanée et hydratation, Peau grasse, hyperséborrhée et séborégulation

La glande sébacée est un organe localisé au niveau du derme. Elle est responsable de la synthèse et de la sécrétion du sébum qui entre dans la composition du film hydrolipidique. Le rôle du sébum est de protéger la peau des agressions extérieures et de la déshydratation. Le sébum permet également d’entretenir la souplesse de la peau et des cheveux.

Read more
28 November 2017/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2017/11/Sébocytes-image-à-la-une.jpg 368 701 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2017-11-28 09:24:342019-09-13 07:30:37La glande sébacée : Physiologie et fonctions

New biological effects of Aphloïol

Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration

In the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.

Read more
24 March 2017/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2017/03/KRT10-Vit-C-_-2017-03-23.jpg 567 800 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2017-03-24 12:03:322019-09-13 21:35:41New biological effects of Aphloïol

 

 

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Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
© Copyright 2019 - Bioalternatives - Legal disclaimer
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