Bioalternatives has many in vitro or ex vivo models at your disposal:
- normal human epidermal keratinocytes (NHEK)
- reconstructed human epidermis (RHE)
- sebocytes (SEBO662AR)
- full thickness skin (FTSK)
- skin explants (ex vivo)
on which we can evaluate case by case the hydrating effect of active compounds or formulations by measuring:
- the reinforcement of the barrier function
- the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
- lipid synthesis (acid mantle):
- sebaceous lipids
- epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
- the expression or synthesis of epidermal extracellular matrix components:
- glycosaminoglycans and hyaluronic acid
- proteoglycans and ECM receptors
- proteases (e.g. MMPs)
- the expression of markers of epidermal cohesion and intercellular cell junctions:
- occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
- dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
- gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
Here are a few examples among all standard assays proposed by Bioalternatives in the field of hydration and reinforcement of the barrier function:
HYDRATION AND REINFORCEMENT OF THE SKIN BARRIER FUNCTION: CLINICAL BIOANALYSIS
Analysis of lipids involved in the barrier function
Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.
The epidermal lipids involved in the barrier function of the epidermis (ceramides, fatty acids and cholesterol) are removed using the SW Kit.
The analysis of these lipids makes it possible to evaluate the quality of the intercorneocyte cement involved in the barrier function of the epidermis and in the prevention of transepidermal water loss (TEWL).
These evaluations help support your claims about the efficacy of biomimetic products, barrier products, protective products, moisturizers, etc.
Analysis of the components of the Natural Moisturizing Factor (NMF)
The amino acids and minerals present on the surface of the skin are collected using the SW Kit:
- PCA / UCA (cis/trans) – (catabolites of filaggrin)
- Amino acids
- Urea, lactates
- Mineral elements: Ca, K, Na, Mg, Zn, etc.
The analysis of these compounds makes it possible to evaluate the impact of the NMF component on skin hydration.
These analysis help support your claims about the efficacy of hygroscopic products, barrier products, protective products, moisturizers, etc.
Ceramide screening – LC/MS
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Damaged and healthy corneocytes – SEMX500
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PCA analysis – LC/UV
New Biological Activities of Lythrum salicaria L.
/in Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration /by JulieThe perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
La glande sébacée : Physiologie et fonctions
/in Acné, Barrière cutanée et hydratation, Peau grasse, hyperséborrhée et séborégulation /by GuillaumeLa glande sébacée est un organe localisé au niveau du derme. Elle est responsable de la synthèse et de la sécrétion du sébum qui entre dans la composition du film hydrolipidique. Le rôle du sébum est de protéger la peau des agressions extérieures et de la déshydratation. Le sébum permet également d’entretenir la souplesse de la peau et des cheveux.
New biological effects of Aphloïol
/in Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydration /by GuillaumeIn the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.