Bioalternatives has many in vitro or ex vivo models at your disposal:
- sebocyte cell line (SEBO662)
- 3D sebocyte cell line (SEBO662 3D)
- sebocyte cell line in response to androgens (SEBO662AR)
on which we can evaluate the effects of active ingredients or cosmetic formulations on sebaceous gland regulation and acne by measuring:
- sebocyte differentiation and maturation (EMA, KRT7, etc.)
- lipidogenesis (lipid accumulation and production)
- 5-alpha reductase activity and testosterone metabolism
Here are a few examples among all assays proposed by Bioalternatives in the field of oily skin and hyperseborrhea:
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
/in Cosmetics, Sebaceous gland regulation, Skin protection and immune defense system /by Linhda CoulevardSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.
Acne vulgaris: physiopathology and cell mechanisms
/in Acne, Sebaceous gland regulation /by JulieAcne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization of the pilosebaceous follicle by certain bacteria (P. acnes).
Bioalternatives has developed a panel of innovative assays to discover and evaluate compounds for the treatment of acne.
Physiology and functions of the sebaceous gland
/in Acne, Sebaceous gland regulation, Skin barrier and hydration /by JulieThe sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.
Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor
/in Acne, Cell and tissue engineering, Sebaceous gland regulation /by GuillaumeIn order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1…) known to be androgen-sensitive in other androgenic cells and tissues.
Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium
/in Acne, Cell and tissue engineering, Sebaceous gland regulation /by GuillaumeSEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.