Oily skin and hyperseborrhea

Oily skin is characterized by the accumulation on its surface of excess sebum, a substance secreted by sebaceous glands. Sebum is secreted by sebocytes through a process of cell differentiation and lipid synthesis or accumulation, called lipidogenesis.

Lipidogenesis is dependent on many factors (hormones, food, pharmaceutical active ingredients, xenobiotics, etc.), which can be identified in in vitro experiments. Mature sebocytes undergo apoptosis and burst to release sebum, which travels through the hair follicle to the skin surface. Sebum lubricates the hair follicle and spreads over the surface of the skin at the stratum corneum level.

An overproduction of sebum, called hyperseborrhea, is responsible for oily skin and greasy hair. In some cases, hyperseborrhea can also contribute to the development of acne prone skin.

Oily skin and hyperseborrhea: in vitro models and assays

Bioalternatives has many in vitro or ex vivo models at your disposal:

sebocyte cell line (SEBO662)
3D sebocyte cell line (SEBO662 3D)
sebocyte cell line in response to androgens (SEBO662AR)

on which we can evaluate the effects of active ingredients or cosmetic formulations on sebaceous gland regulation and acne by measuring:

sebocyte differentiation and maturation (EMA, KRT7, etc.)
lipidogenesis (lipid accumulation and production)
5-alpha reductase activity and testosterone metabolism

SEBO662-3D-(-lipide-en-rouge—Oil-Red-O)) — 3D sebocytes (SEBO662) (Oil Red O staining)

SEBO662-(lipides-en-vert—bodipy) — Labeling of sebocytes (SEBO662) (Bodipy in green, nuclei in blue)

mf_64-Labeling-of-sebocytes-(SEBO662)-(Bodipy-in-green,-cytokeratin-7-in-red,-nuclei-in-blue) — Labeling of sebocytes (SEBO662) (Bodipy in green, cytokeratin 7 in red, nuclei in blue)

ImageJ=1.49j
unit=um — Labeling of SEBO662AR cells treated by DHT (Bodipy in green, EMA in red and nuclei in blue)

Here are a few examples among all assays proposed by Bioalternatives in the field of oily skin and hyperseborrhea:

Discover all our standard assays related to oily skin and hyperseborrhoea

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Oily skin and hyperseborrhea: posts and publications

Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract

Sebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.

Acne vulgaris: physiopathology and cell mechanisms

29 November 2017/in Acne, Sebaceous gland regulation /by Julie

Acne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization of the pilosebaceous follicle by certain bacteria (P. acnes).
Bioalternatives has developed a panel of innovative assays to discover and evaluate compounds for the treatment of acne.

Physiology and functions of the sebaceous gland

28 November 2017/in Acne, Sebaceous gland regulation, Skin barrier and hydration /by Julie

The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.

Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor

9 April 2015/in Acne, Cell and tissue engineering, Sebaceous gland regulation /by Guillaume

In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1…) known to be androgen-sensitive in other androgenic cells and tissues.

Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium

24 January 2012/in Acne, Cell and tissue engineering, Sebaceous gland regulation /by Guillaume

SEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.

Oily skin and hyperseborrhea: in vitro models and assays

SEBO662AR, sebocyte differentiation (testosterone stimulation)

SEBO662AR, lipogenesis (testosterone stimulation)

SEBO662AR, lipogenesis (lipogenic and androgenic factor stimulation)

SEBO662AR, apolar lipid neosynthesis (testosterone stimulation)

SEBO662AR, testosterone metabolism & 5- alpha reductase activity (basal)

Oily skin and hyperseborrhea: posts and publications

Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract

Acne vulgaris: physiopathology and cell mechanisms

Physiology and functions of the sebaceous gland

Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor

Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium

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