Bioalternatives has many in vitro models at your disposal:
- intrinsic ageing model:
- “aged” human dermal fibroblasts (Hayflick model)
- “aged” dermal equivalent (Hayflick model)
- accelerated ageing model:
- human dermal fibroblasts aged by oxidative stress (H2O2)
- human reconstructed skin in deficient medium
- photo-ageing model:
- human dermal fibroblasts subjected to UVA, infrared or UV radiation
- photo-aged human full thickness reconstructed skin
on which we can evaluate the anti-ageing effect of active cosmetic compounds on:
- cell renewal (cell proliferation, migration and differentiation)
- extracellular matrix synthesis and degradation (collagen, elastin, hyaluronic acid, MMPs, etc.)
- senescence marker expression
- free radical production
Here are a few examples among all standard assays proposed by Bioalternatives in the field of skin ageing:
Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
/in Cell and tissue engineering, Skin ageing /by GuillaumeThe development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
/in Cosmetics, Cosmétique, Skin ageing /by GuillaumeThese data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
/in Cosmetics, Cosmétique, Skin ageing /by Guillaumetensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing