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Bioalternatives
  • Bioalternatives
    • About us
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    • Careers
  • Cosmetics
    • Hydration and skin barrier
    • Epidermal regeneration
    • Skin firmness & cohesion
    • Skin ageing
    • Skin protection & defense
    • Skin pigmentation
    • Oily skin and hyperseborrhea
    • Skin microbiota
    • Slimming and adipocyte metabolism
    • Skin microcirculation and vascularization
    • Hair growth, alopecia & canities
  • Dermatology
    • Acne
    • Skin microbiota
    • Alopecia, hair growth & canities
    • Atopic dermatitis
    • Wound healing and skin regeneration
    • Psoriasis
    • Melanoma
    • Vitiligo (coming soon)
  • Pharmacology
    • Immuno-Inflammation
    • Veterinary medicine
    • Neurobiology
  • Bioanalysis and bioengineering
    • Analytical and bioanalytical services
      • Bioanalysis of non-invasive clinical samples
    • Cell and tissue engineering
    • Chemoinformatics, in silico virtual screening & phytochemical profiling
      • in silico screening – SELNERGY
      • Phytochemical analysis & metabolic profiling – GAINS
    • Gene expression and transcriptomic analysis
      • RNA extraction and quality control
      • Rt-qPCR & PCRarrays
      • Microarrays
      • miRNA
      • In situ hybridization
      • Bioinformatics
    • Histology services
      • Slide preparation
      • Histological staining
      • Immunolabeling
      • Electron microscopy TEM/SEM
      • Slide scanning
    • Development and validation of assays and protocols
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SKIN AGEING

Skin ageing is a natural, multifactorial and still little-known process which results both from intrinsic factors (genomic, hormonal, etc.) and from external aggravating factors (UV radiation, pollution, food, free radicals, etc.). The latter are referred to as accelerated ageing or as photo-ageing in the particular case of UVs.

Intrinsic ageing and accelerated ageing have slightly different phenotypic characteristics. Intrinsic ageing is characterized by skin dryness, fine wrinkles and a loss of adipose tissue. Photo ageing is characterized by a significant loss of firmness and elasticity (loose skin), deeper wrinkles and pigmentation disorders.

In the past few years, significant progress has been made in the understanding of cell ageing mechanisms and we can now explore new dermo-cosmetics strategies which might prevent or decelerate the signs of ageing.

Skin ageing: in vitro models and assays

Bioalternatives has many in vitro models at your disposal:

  • intrinsic ageing model:
    • “aged” human dermal fibroblasts (Hayflick model)
    • “aged” dermal equivalent (Hayflick model)
  • accelerated ageing model:
    • human dermal fibroblasts aged by oxidative stress (H2O2)
    • human reconstructed skin in deficient medium
  • photo-ageing model:
    • human dermal fibroblasts subjected to UVA, infrared or UV radiation
    • photo-aged human full thickness reconstructed skin

on which we can evaluate the anti-ageing effect of active cosmetic compounds on:

  • cell renewal (cell proliferation, migration and differentiation)
  • extracellular matrix synthesis and degradation (collagen, elastin, hyaluronic acid, MMPs, etc.)
  • senescence marker expression
  • free radical production

Here are a few examples among all standard assays proposed by Bioalternatives in the field of skin ageing:

  • MMP1 gene expression

    NHDF, gene expression (UVA stimulation)

    1.00€
    Add to cart Show Details
  • ELN gene expression

    “Aged” fibroblasts, control of aging (basal)

    1.00€
    Add to cart Show Details
  • MMP-1 release

    Fibroblasts, prevention of aging, procollagen I and/or MMP-1 synthesis (H2O2 stimulation)

    1.00€
    Add to cart Show Details
  • Hyaluronic acid synthesis #

    “Aged” fibroblasts, control of aging, hyaluronic acid synthesis (basal)

    1.00€
    Add to cart Show Details
  • Procollagen I release

    “Aged” fibroblasts, reversion of aging, procollagen I and/or MMP-1 synthesis (basal)

    1.00€
    Add to cart Show Details

Skin ageing: clinical bioanalysis

Analysis of lipids involved in the barrier function

Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.

The epidermal lipids involved in the barrier function of the epidermis (ceramides, fatty acids and cholesterol) are removed using the SW Kit.

The analysis of these lipids makes it possible to evaluate the quality of the intercorneocyte cement involved in the barrier function of the epidermis and in the prevention of transepidermal water loss (TEWL).

These evaluations help support your claims about the efficacy of biomimetic products, barrier products, protective products, moisturizers, etc.

Analysis of the components of the Natural Moisturizing Factor (NMF)

The amino acids and minerals present on the surface of the skin are collected using the SW Kit:

  • PCA / UCA (cis/trans) – (catabolites of filaggrin)
  • Amino acids
  • Urea, lactates
  • Mineral elements: Ca, K, Na, Mg, Zn, etc.

The analysis of these compounds makes it possible to evaluate the impact of the NMF component on skin hydration

These analysis help support your claims about the efficacy of hygroscopic products, barrier products, protective products, moisturizers, etc.

Analysis of markers of oxidative stress

Oxidative stress is also a factor of accelerated skin ageing. Oxidative stress markers are analyzed from samples using the SW Kit. The sampling areas depend on the type of stress (induced stress or external environment).

The analyzed markers are:

  • products from peroxidation and lipid detoxification (MDA, peroxidized squalene, CAT, SOD, etc.)
  • protein oxidation products (AOPP Dityrosin, ROH, etc.)

These evaluations help support your claims about the efficacy of anti-pollution products, protective products , anti-aging products, etc.

Screening des céramides

Ceramide screening – LC/MS

–

Cornéocytes endommagés et sains - SEMX500

Damaged and healthy corneocytes – SEMX500

–

Analyse des PCA - LC/UV

PCA analysis – LC/UV

Find out about all our standard assays - Direct access to catalog
A project, an idea or a question? Contact us and let’s talk about it!

Skin ageing: posts and publications

skin photoaging irradiations

in vitro modeling of skin photoaging: development of evaluation tools for cosmetics

Cosmetics, Skin ageing

Development of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.

Read more
21 March 2017/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2017/03/Silab-Fig-2-2017-03-21.jpg 242 635 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2017-03-21 18:10:582019-09-13 21:45:10in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
ageing reconstructed epidermis

Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds

Cell and tissue engineering, Skin ageing

The development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.

Read more
21 December 2016/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/12/Epiderme-reconstruit-passage-2-figure-1A-1.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2016-12-21 16:21:562019-09-13 21:40:06Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds

Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study

Cosmetics, Cosmétique, Skin ageing

These data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.

Read more
24 March 2011/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Effect-of-C-xyloside.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2011-03-24 10:58:512019-11-13 11:34:13Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
tensin1

Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction

Cosmetics, Cosmétique, Skin ageing

tensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing

Read more
4 June 2008/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Tensin1.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2008-06-04 16:13:102019-11-13 11:34:03Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction

 

 

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Address

QIMA US.
Buffalo, NY, USA
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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