Bioalternatives has many in vitro models at your disposal:
- intrinsic ageing model:
- “aged” human dermal fibroblasts (Hayflick model)
- “aged” dermal equivalent (Hayflick model)
- accelerated ageing model:
- human dermal fibroblasts aged by oxidative stress (H2O2)
- human reconstructed skin in deficient medium
- photo-ageing model:
- human dermal fibroblasts subjected to UVA, infrared or UV radiation
- photo-aged human full thickness reconstructed skin
on which we can evaluate the anti-ageing effect of active cosmetic compounds on:
- cell renewal (cell proliferation, migration and differentiation)
- extracellular matrix synthesis and degradation (collagen, elastin, hyaluronic acid, MMPs, etc.)
- senescence marker expression
- free radical production
Here are a few examples among all standard assays proposed by Bioalternatives in the field of skin ageing:
Skin ageing: clinical bioanalysis
Analysis of lipids involved in the barrier function
Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.
The epidermal lipids involved in the barrier function of the epidermis (ceramides, fatty acids and cholesterol) are removed using the SW Kit.
The analysis of these lipids makes it possible to evaluate the quality of the intercorneocyte cement involved in the barrier function of the epidermis and in the prevention of transepidermal water loss (TEWL).
These evaluations help support your claims about the efficacy of biomimetic products, barrier products, protective products, moisturizers, etc.
Analysis of the components of the Natural Moisturizing Factor (NMF)
The amino acids and minerals present on the surface of the skin are collected using the SW Kit:
- PCA / UCA (cis/trans) – (catabolites of filaggrin)
- Amino acids
- Urea, lactates
- Mineral elements: Ca, K, Na, Mg, Zn, etc.
The analysis of these compounds makes it possible to evaluate the impact of the NMF component on skin hydration
These analysis help support your claims about the efficacy of hygroscopic products, barrier products, protective products, moisturizers, etc.
Analysis of markers of oxidative stress
Oxidative stress is also a factor of accelerated skin ageing. Oxidative stress markers are analyzed from samples using the SW Kit. The sampling areas depend on the type of stress (induced stress or external environment).
The analyzed markers are:
- products from peroxidation and lipid detoxification (MDA, peroxidized squalene, CAT, SOD, etc.)
- protein oxidation products (AOPP Dityrosin, ROH, etc.)
These evaluations help support your claims about the efficacy of anti-pollution products, protective products , anti-aging products, etc.
Ceramide screening – LC/MS
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Damaged and healthy corneocytes – SEMX500
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PCA analysis – LC/UV
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
Cosmetics, Skin ageingDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
Cell and tissue engineering, Skin ageingThe development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
Cosmetics, Cosmétique, Skin ageingThese data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
Cosmetics, Cosmétique, Skin ageingtensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing