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Bioalternatives
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    • Hydration and skin barrier
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    • Skin microbiota
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    • Vitiligo (coming soon)
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SKIN MICROBIOTA

Download a sample report

What is skin microbiota?

The skin microbiota is an ecosystem inhabited by a community of more than a million microorganisms (bacteria, viruses, fungi) per square centimeter of skin.

Bacterial composition of skin (cutaneous microbiota) is mostly dominated by the following genera: Propionibacteria (23%), Corynebacteria (22.8%) and staphylococci (16.8%).

Most of the bacteria in epidermis are “commensal” and live in a symbiotic manner with the host that they colonize.

Interactions between the host immune system and its microbiota contribute to the control of the skin bacterial composition as well as to the education of the adaptive immune system of the host.

Imbalance in the microbiota composition can cause cutaneous disorders (dry skin, oily skin, dandruff, etc.) and even pathologies (psoriasis, atopic dermatitis, acne, etc.).

S.aureus on RHE_SEMX2000

The skin microbiota: models and in vitro assays

Thanks to its expertise in cellular and tissue engineering, Bioalternatives has developed in vitro or ex vivo study models that are suitable for analyzing the interactions between microbiota and skin:

  • Isolated bacterial culture (Staphylococcus aureus, Staphylococcus epidermidis, Cutibacterium acnes, Corynebacterium xerosis …)
  • Co-culture:
    • bacteria on 2D skin models (keratinocytes, fibroblasts, sebocytes, etc.)
    • bacteria on 3D skin models (reconstructed epidermis, etc.)
  • infection experiments with the HSV-1 virus ( Herpes simplex virus type I)

Both the impact of compounds or formulations on the development of bacteria and the influence of bacteria on cutaneous response can be assessed by these models.

Bioalternatives offers a panel of solutions for the evaluation of:

  • Bacterial viability
  • Quantification of bacterial colony (CFU)
  • Determination of bacterial load (qPCR (Taqman))
  • Bacterial adhesion on 3D models (reconstucted epidermis) – quantification of colony, qPCR, radioactivity, SEM
  • Biological response of 2D or 3D models (differentiation, inflammation and immune response)

Bioalternatives offers a wide range of microorganisms tested by our laboratory and available for in vitro testing:

BACTERIA

GRAM (+) :

  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Cutibacterium acnes
  • Corynebacterium xerosis
  • Streptococcus pyogenes
  • Mix of several strains (upon request)
  • Other strains upon request

GRAM (-) :

  • Pseudomonas aeruginosa
  • Other strains upon request

VIRUSES

  • Herpes simplex virus type 1 (HSV-1)
  • Human Rhinovirus 16 (HRV-16)

YEASTS

Coming soon

Here are a few examples among all standard assays proposed by Bioalternatives in the field of skin microbiota:

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  • BACT-0001_Bacterial enumeration #1

    Bacterial enumeration

    1.00€
    Add to cart Show Details
  • BACT-0007_Induction of a bacterial resistance

    Induction of a bacterial resistance (mutant strain emergence)

    1.00€
    Add to cart Show Details
  • BACT-0008

    Minimum Inhibitory Concentration determination regarding a bacterial strain

    1.00€
    Add to cart Show Details
  • NHDFBACT-0001_IL-8 release#Stim

    NHDF, Cytokine/chemokine release (C. acnes stimulation)

    1.00€
    Add to cart Show Details
  • NHDFBACT-0003_IL-8 release#Stim

    NHDF, Cytokine/chemokine release (S.aureus stimulation)

    1.00€
    Add to cart Show Details
  • EPIBACT-0002_Protocol_Schema

    RHE, bacterial adhesion of Staphylococcus aureus (basal)

    1.00€
    Add to cart Show Details
  • SEBOARBACT-0001_IL-8 release#Stim

    SEBO662AR, Cytokine/chemokine release (C. acnes stimulation)

    1.00€
    Add to cart Show Details
  • SEBOARBACT-0004_IL-8 release#Stim

    SEBO662AR, Cytokine/chemokine release (S.aureus stimulation)

    1.00€
    Add to cart Show Details
Skin microbiota analysis

Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.

The qualitative and quantitative analysis of the bacteria present on the surface of the skin are carried out on samples with the SW Kit. They are performed using the following techniques:

  • Traditional microbiology on agar
  • Targeted qPCR
  • Non-targeted metagenomic analysis

In addition, the analysis of sebaceous lipids (triglycerides and fatty acids) gives information on the lipase activities of bacteria and yeasts such as C. acnes and M. furfur, involved in the development of acne and dandruff respectively.

These evaluations help support your claims about the efficacy of “friendly” microbiota products, bacteriostats, antimicrobials, prebiotics, anti-acne products, anti-dandruff products, etc.

Quantification bactérienne sur échantillons clinique

Find out about all our standard assays - Direct access to catalog
A project, an idea or a question? Contact us and let’s talk about it!

Skin microbiota: posts and publications

BACT-0003_Bacterial growth #1

The Relationship Between Microbiology Testing and the Skin Microbiota

Cosmetics, Skin microbiome

Microbiology testing is essential for substantiating microbiome-friendly and anti-microbial claims. Read on to find out how cosmetic microbiology testing is connected to the skin microbiome.

Read more
23 January 2020/by Vanessa
https://www.bioalternatives.com/wp-content/uploads/2019/08/5897-1.jpg 453 640 Vanessa https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Vanessa2020-01-23 14:58:062020-01-23 14:58:06The Relationship Between Microbiology Testing and the Skin Microbiota
BACT-0003_Bacterial growth #1

Microbiota vs. Microbiome: What’s the Difference?

Skin microbiome

What is the difference between microbiota vs. microbiome? Why are they important to human skin? What is the Skin Microbiota test? Learn more here.

Read more
14 January 2020/by Vanessa
https://www.bioalternatives.com/wp-content/uploads/2019/08/5897-1.jpg 453 640 Vanessa https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Vanessa2020-01-14 16:05:162020-06-11 17:13:27Microbiota vs. Microbiome: What’s the Difference?

Using Reconstructed Human Epidermis model in Skin Microbiome Research

Skin microbiome, Skin microbiome

Skin microbiome research is developing rapidly. Are you up to date? Find out why 3D skin models are an important development in skin microbiome research.

Read more
11 December 2019/by Vanessa
https://www.bioalternatives.com/wp-content/uploads/2019/03/190328_Vignettes_Microbiome_EN.jpg 368 655 Vanessa https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Vanessa2019-12-11 11:45:282019-12-11 12:26:10Using Reconstructed Human Epidermis model in Skin Microbiome Research

Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation

Cosmetics, Microbiome cutané, Skin microbiome

In this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains…

Read more
8 October 2019/by Vanessa
https://www.bioalternatives.com/wp-content/uploads/2019/10/191007_IFSCC2019_Poster_Figure-intro.jpg 357 1092 Vanessa https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Vanessa2019-10-08 14:42:142019-11-12 17:57:11Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation

 

 

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Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
© Copyright 2019 - Bioalternatives - Legal disclaimer
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