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Bioalternatives
  • Bioalternatives
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  • Cosmetics
    • Hydration and skin barrier
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    • Oily skin and hyperseborrhea
    • Skin microbiota
    • Slimming and adipocyte metabolism
    • Skin microcirculation and vascularization
    • Hair growth, alopecia & canities
  • Dermatology
    • Acne
    • Skin microbiota
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    • Atopic dermatitis
    • Wound healing and skin regeneration
    • Psoriasis
    • Melanoma
    • Vitiligo (coming soon)
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    • Analytical and bioanalytical services
      • Bioanalysis of non-invasive clinical samples
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      • in silico screening – SELNERGY
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PROTECTION & SKIN DEFENSES

Download a sample report

Every day, skin and hair are subjected to many stress and environmental factors such as pollution, heat, radiation (UV, infrared, etc.), chemical products or microorganisms (bacteria, yeasts, etc.). As these external aggressions are considered as factors of oxidative stress, inflammation or DNA damages, they can alter skin quality, increase skin sensitivity and lead to premature ageing.

To fight these aggressions, the skin has innate defense and protection mechanisms, on which an active ingredient or cosmetic compound can have an effect: pH, commensal flora, sebum, antimicrobial substances, antioxidant enzymes, detoxification enzymes, immune cells, etc.

Skin protection and defense: in vitro models and assays

Bioalternatives has many in vitro or ex vivo models at your disposal:

  • normal human epidermal keratinocytes (NHEK)
  • normal human dermal fibroblasts (NHDF)
  • reconstructed human epidermis (RHE)
  • normal, photo-aged and deficient human full thickness reconstructed skin (FTSK)
  • skin explants (ex vivo)

on which we can evaluate the protective effect of active ingredients or formulations on:

  • infrared, UVB, UVB+(A) radiation
  • free radicals
  • pollution (urban dust, indoor dust, cigarette smoke, etc.)
  • chemical stress (PMA, SDS, etc.)
  • microorganisms (bacteria, yeasts, viruses, etc.) or pathogen-associated molecular patterns (TLR agonists, etc.)
  • osmotic stress, thermal stress, etc.
  • nutrient deficiency

by measuring (for example):

  • cell viability or metabolism
  • inflammatory mediator release (IL-8, PGE2, IL-6, TNF-alpha, etc.)
  • free radical production
  • DNA damages (CPD, sunburn cells, comet assay, etc.)
  • metalloproteinase expression or production
  • antimicrobial peptide production (beta-defensins, S100A7, etc.)

Here are a few examples among all assays proposed by Bioalternatives in the field of skin protection and defense:

  • Stim

    NHDF, DNA damage (comet assay, H2O2 stimulation)

    1.00€
    Add to cart Show Details
  • Cell viability#Stim

    NHEK, cell viability (protection against indoor dust (1% lead))

    1.00€
    Add to cart Show Details
  • Lipid peroxide content

    NHEK, lipid peroxide content (UV stimulation)

    1.00€
    Add to cart Show Details
  • Stim_Urban dust_Vert

    NHEK, mitochondrial membrane potential (JC-1 Dye labeling) (protection against urban dust)

    1.00€
    Add to cart Show Details
  • ROS content

    NHEK, reactive oxygen species content (H2O2 stimulation)

    1.00€
    Add to cart Show Details

Skin protection and defense: clinical bioanalysis

Analysis of markers of oxidative stress and inflammation

Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.

Oxidative stress and inflammation markers are analyzed from samples using the SW Kit. The sampling areas depend on the type of stress (induced stress or external environment).

The analyzed markers are:

  • products from peroxidation and lipid detoxification (MDA, peroxidized squalene, CAT, SOD, etc.)
  • protein oxidation products (AOPP Dityrosin, ROH, etc.)
  • markers of inflammation (cytokines, cascade of fatty acids, PGE2)

These evaluations help support your claims about the efficacy of anti-pollution products, protective products , anti-aging products, etc. .

Quantification de la SOD

SOD quantification

Screening des marqueurs de l'inflammation

Screening of inflammation markers

Contaminant analysis by Scanning Electron Microscopy (SEM)

Our company offers to evaluate the cleaning and protective effect of products by scanning electron microscopy. Observations have been made on reconstructed human epidermis subjected to pollutants and observed before and after application of the cleaning product.

RHE subject to contaminants before and after cleaning – SEM X 70

Find out about all our standard assays - Direct access to catalog
A project, an idea or a question? Contact us and let’s talk about it!

Skin protection and defense: posts and publications

in vitro and ex vivo assays: skin protection and immune defense system

Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene

Cosmetics, Cosmetics, Cosmétique, Skin protection and immune defense system

In this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.

Read more
17 March 2019/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_AdobeStock_bannierePROTECTION-DEFENSE.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2019-03-17 11:20:102019-09-13 21:46:38Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene
lipid

Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract

Cosmetics, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulation, Skin protection and immune defense system

Sebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.

Read more
29 November 2017/by Guillaume
https://www.bioalternatives.com/wp-content/uploads/2017/11/SEPPIC-Image-à-la-une.jpg 640 1140 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2017-11-29 10:57:192019-09-13 07:39:35Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract

 

 

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Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
© Copyright 2019 - Bioalternatives - Legal disclaimer
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