Bioalternatives has many in vitro or ex vivo models at your disposal:
- normal human epidermal keratinocytes (NHEK)
- normal human dermal fibroblasts (NHDF)
- reconstructed human epidermis (RHE)
- normal, photo-aged and deficient human full thickness reconstructed skin (FTSK)
- skin explants (ex vivo)
on which we can evaluate the protective effect of active ingredients or formulations on:
- infrared, UVB, UVB+(A) radiation
- free radicals
- pollution (urban dust, indoor dust, cigarette smoke, etc.)
- chemical stress (PMA, SDS, etc.)
- microorganisms (bacteria, yeasts, viruses, etc.) or pathogen-associated molecular patterns (TLR agonists, etc.)
- osmotic stress, thermal stress, etc.
- nutrient deficiency
by measuring (for example):
- cell viability or metabolism
- inflammatory mediator release (IL-8, PGE2, IL-6, TNF-alpha, etc.)
- free radical production
- DNA damages (CPD, sunburn cells, comet assay, etc.)
- metalloproteinase expression or production
- antimicrobial peptide production (beta-defensins, S100A7, etc.)
Here are a few examples among all assays proposed by Bioalternatives in the field of skin protection and defense:
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Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene
/in Cosmetics, Cosmetics, Cosmétique, Skin protection and immune defense system /by GuillaumeIn this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
/in Cosmetics, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulation, Skin protection and immune defense system /by GuillaumeSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.