Bioalternatives has many in vitro or ex vivo models at your disposal:
- normal human epidermal keratinocytes (NHEK)
- normal human dermal fibroblasts (NHDF)
- reconstructed human epidermis (RHE)
- normal, photo-aged and deficient human full thickness reconstructed skin (FTSK)
- skin explants (ex vivo)
on which we can evaluate the protective effect of active ingredients or formulations on:
- infrared, UVB, UVB+(A) radiation
- free radicals
- pollution (urban dust, indoor dust, cigarette smoke, etc.)
- chemical stress (PMA, SDS, etc.)
- microorganisms (bacteria, yeasts, viruses, etc.) or pathogen-associated molecular patterns (TLR agonists, etc.)
- osmotic stress, thermal stress, etc.
- nutrient deficiency
by measuring (for example):
- cell viability or metabolism
- inflammatory mediator release (IL-8, PGE2, IL-6, TNF-alpha, etc.)
- free radical production
- DNA damages (CPD, sunburn cells, comet assay, etc.)
- metalloproteinase expression or production
- antimicrobial peptide production (beta-defensins, S100A7, etc.)
Here are a few examples among all assays proposed by Bioalternatives in the field of skin protection and defense:
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
/in Cosmetics, Sebaceous gland regulation, Skin protection and immune defense system /by Linhda CoulevardSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.