Facilitating skin healing, minimizing the aesthetic impact on the patient and restoring optimal tissue function remain wound healing management concerns for the dermatological and cosmetic industry.
Wound healing is a complex and multi-step process based on the dialogue between numerous cellular actors.
A good knowledge and understanding of the cellular and molecular mechanisms of the healing process participating in skin regeneration are fundamental for the development of wound care therapies (APIs, biosimilars, formulations, medical devices). Bioalternatives can provide you with more than 20 years of experience in skin biology and in vitro testing. Here are some of the approaches we offer in the field of wound healing and skin regeneration for the evaluation of wound healing products.
Wound healing and skin regeneration: in vitro models and assays
Bioalternatives has many in vitro or ex vivo models at your disposal:
- Cell lines
- Peripheral blood immune cells (PBMCs and purifed blood cell populations: subpopulations of T lymphocytes, monocytes, polymorphonuclear cells, etc.)
- Macrophages and monocyte-derived dendritic cells
- Normal human epidermal keratinocytes (NHEK)
- Normal human dermal fibroblasts (NHDF)
- Mesenchymal stem cells
- Microvascular endothelial cells
- Normal human epidermal melanocytes (NHEM)
- Sensory neurons
- Reconstructed human epidermis (RHE)
- Dermal equivalents
- Skin explants (ex vivo)
on which we can evaluate the pro-healing effect of active compounds, formulations or medical devices by measuring:
- Inflammatory response, cleaning of skin wounds and innate immunity
- Neovascularization and neuritogenesis
- Extracellular matrix synthesis, fibrosis and myofibroblast interaction
- Dermal remodeling and epidermal differentiation
Among all the standard assays proposed by Bioalternatives in the field of wound healing and skin regeneration, here are a few examples: