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2D & 3D in vitro canine models

Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Bioalternatives has several canine in vitro models such as canine primary cells, canine 2D&3D in vitro models and in vitro models of canine whole blood.

We have also developed models and protocols suitable for modeling pathologies (inflammation, infections, senescence, degeneration, atopic dermatitis) or aggressions (stress, wound healing).

We are presenting here some results from a study conducted on canine whole blood model.

Please, do not hesitate to contact us for more information.

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Objective of the study using canine whole blood

The objective of this study was to evaluate the anti-inflammatory and immunomodulatory effects of a product (at different concentrations: C1 / C2 / C3) on the production of IFNg (T-lymphocyte activity) and on the activation of COX-2 (PGE2) and 5-LOX (LTB4) pathways using in vitro canine whole blood models.

The study was performed on whole blood of 5 dogs.

1 – Canine whole blood parameters

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Blood cell counts and other blood parameters were measured using ABC Vet with dog Smart card.

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2 – Evaluation of T-Lymphocytes activity

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  • In the absence of stimulation, very low amounts of IFNg were produced (< LOQ)
  • Stimulation with PHA increased IFNg  release (> 1000 pg/ml)
  • Pre-treatment with the product had a slightly effect on PHA-induced IFNg release

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3 – Evaluation of Cox-2 activity

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  • In the absence of stimulation, very low amounts of PGE2 were produced
  • Stimulation with LPS increased PGE2 release (> 100000 pg/ml)
  • Pre-treatment with the product inhibited PGE2 production in a dose dependent manner and was as effective as the reference at the highest concentration (C3)

4 – Evaluation of 5-Lox activity

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  • In the absence of stimulation, low amounts of LTB4 were produced
  • Stimulation with A23187 increased LTB4 release (> 90000 pg/ml)
  • Pre-treatment with the product product inhibited LTB4 production in a dose dependent manner

Conclusion – Mean inhibition

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  • Blood cells were functional as they produced PGE2, IFNg and LTB4 in response to stimuli
  • For all the tested stimuli, references inhibited more than 80% of the response for all dogs
  • The product is very efficient at reducing inflammation in dog while maintaining normal T cell mediated responses

Evaluation of mean inhibition

Do you need more information regarding this study? Do you want to evaluate the efficacy of your products using whole blood?
Let’s talk about it!

CONTACT US

See also

3 November 2020

Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs

Evaluation of mean inhibition - canine whole bool model
21 July 2020

Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

in vitro canine models
16 July 2020

2D and 3D in vitro canine models

Pro-Filaggrin Expression - atopic reconstructed canine epidermis
16 July 2020

Evaluation of the effect of a reference molecule on an Atopic Reconstructed Canine Epidermis model (RCE-AD)

25 May 2020

A prospective, randomized, double blind, placebo-controlled evaluation of the effects of an n-3 essential fatty acids supplement (Agepi® ω3) on clinical signs, and fatty acid concentrations in the erythrocyte membrane, hair shafts and skin surface of dogs with poor quality coats

 

 

 

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Address

Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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