HISTOLOGY

IMMUNOHISTOCHEMISTRY

IMMUNOFLUORESCENCE

As a researcher in the pharmaceutical industry or as a physician, you wish to carry out histological or immunohistochemical analysis as part of your research program or as part of your preclinical or clinical studies.
Do you have samples (tissues, human or animal biopsies), blocks or slides that are ready to be analyzed? Are you looking for a reactive high quality contract research organization that can provide you with expertise and flexibility?

Our laboratory and our special team dedicated to histology and immunohistochemistry methods would like to assist you with your studies.

Routine analysis

  • Preparation of samples to study:
    • cryo blocks or paraffin inclusion
    • generation of slices
  • Methods:
    • trichrome staining, specific staining
    • immunolabeling (immunofluorescence or immunohistochemistry)
  • Microscope observation and  generation of images
  • Image analysis: scoring, quantification
  • Human skin (CD44 and cytokeratin-15 immunolabeling)

  • Hair-sebaceous unit (Orcein and Saffron staining)

  • Leica Cryotome

  • Skin explant (Mowry staining)

  • Leica TP1020 automated tissue processor

  • Hair follicle (KRT17 immunolabeling and nuclei)

  • Leica E400 miscroscope and Nikon camera

  • Human skin (fibrillin-1 and collagen I immunolabeling)

  • Leica E400 miscroscope

Customized services

  • Development:
    • staining
    • immunolabeling (research and validation of antibodies)
    • co-labeling
  • Preparation of slices
  • Images, image mosaic, slice scanning
  • Quantification / image analysis (thickness, intensity, counts, etc.)

KEY FIGURES

+24

staining methods

+60

validated antibodies for IHC/IF

+ 4 000

analyzed samples per year

+30 000

pictures per year

Histology, trichrome staining and specific staining

Bioalternatives offers a wide choice of histological staining techniques, known as trichrome staining, to observe morphology (structure, organization and dimension), integrity or possible alterations, in a global way. We can also carry out special staining processes upon request, in order to visualize particular tissues, such as melanin pigments by Fontana Masson, elastin by Luna staining, lipids by Sudan black staining, etc.
You will find some examples of this below:

Immunohistofluorescence

Immunofluorescence is a special in situ detection technique, which consists in detecting and localizing a protein of interest within a tissue section, using a specific antibody (directly linked to a fluorophore or detected by a secondary fluorescent antibody). Immunolabeling of proteins of interest is generally associated to nuclear labeling by Hoechst staining or by propidium iodide staining. This technique, when associated to an image processing program (e.g. ImageJ), can also generate fluorescence intensity data that can be analyzed quantitatively.

Immunohistochemistry

Immunohistochemistry by enzyme detection is the second special in situ detection technique, which consists in detecting and localizing a protein of interest within a tissue section, using a specific antibody, which will be detected by an enzymatic reaction (such as peroxidase or alkaline  phosphatase) that generates a generally brown or blue chromogen product. Co-labeling can also be performed using this technique which remains a qualitative or semi-quantitative method.