in situ hybridization is a technique for visualizing specific RNA or DNA within cell or tissue. Its principle is based on the use of a fluorescent probe which represents a nucleic acid sequence specific to a target-gene.
This technology offers quantitative and visual information about the studied mRNA. It is an alternative to immunohistochemistry or immunofluorescence experiments when there is no available antibody. in situ hybridization can also be of interest for the analysis of specific gene expression profiles in sensitive samples, where total RNA extraction by RT-qPCR may not provide satisfactory results.
Bioalternatives can assist you in the implementation and in the analysis of your in situ hybridization experiments.