Cultured keratinocyte cells from foreskin and future application for burns in children

Annales de chirurgie plastique et esthétique, 54(6):528-32

MCHEIK JN., BARRAULT C., VINCENT G., GRAMMATICO F., PECI S., GARNIER J., BERNARD FX., DEGUERCY A. and LEVARD G. (2009)

Chirurgie pédiatrique, hôpital Jean-Bernard, CHU de Poitiers, France.
BIO
alternatives, Gençay, France.

Abstract

We tested in vitro the keratinocytes capacity for division and differentiation. The donor site was the human foreskin.
For 12 months, we harvested 18 foreskins after circumcision. The middle age of the operated children was four years. The keratinocytes were isolated after double enzymatic digestion (thermolysin and trypsin, respectively). After filtration and centrifugation we put the keratinocytes in culture. In parallel, the keratinocytes were cultivated on the surface of collagen lattices. The keratinocytes were cultured in submerged condition for two days and then in an air-liquid interface condition for further differentiation. After nine days of culture, a histological examination and immunostain were used. An immunohistologic analysis made it possible to highlight the markers characteristic of epidermal skin differentiation.
We obtained an average of 8.8 10(6) cells per foreskin. After seven days of culture, we obtained on average 23.7 10(6) cells by culture. In contact with the collagen lattices, we obtained an epidermal skin and we highlighted the markers of keratinocytes differentiation as well as the markers of the dermoepidermic junction.
The keratinocytes resulting from foreskin have a high capacity of division. These cells can divide a long time before differentiation. The observations enable us to propose with our patients the keratinocytes from foreskin for wound healing especially for burns in children.

© 2008 Elsevier Masson SAS.

KEYWORDS: Keratinocytes; Foreskin; Cultured skin; Experimental surgery

Check out Bioalternatives’ updates and experience new testing ideas

  • Bioassays, models and services
  • Posts and publications
  • Events

Related Posts or publications

Wound healing, granulation phase and maturation phase The third phase of wound healing, consisting in the replacement of the provisional  fibrin matrix with granulation tissue once the wound has been debrided, includes several sub-phases: re-epithelialization, fibroplasia, collagen deposition and angio...
Wound healing, hemostasis phase and inflammatory phase The inflammatory phase is characterized by the sequential infiltration of polymorphonuclear neutrophils (PMNs), monocytes/macrophages and lymphocytes. IL-8 facilitates PMNs migration from surrounding blood vessels.
Wound healing, overview Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells , stem cells , hair follicles, extracellular matrix (ECM) proteins, cytokines networks, mic...
Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children Our studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested ...
Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective In the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor s...