Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract

Catherine KERN1, Erwan LE GÉLÉBART2, Laetitia CATTUZZATO, Sevda CORDIER-DIRIKOC3, Nathalie PEDRETTI3, François-Xavier BERNARD3, Sandy DUMONT1, Carla PEREZ4

1SEPPIC – Innovation Direction – 127 chemin de la Poudrerie BP90228, 81105 Castres cedex, France

lipid

2BiotechMarine – Innovation Direction – ZI BP72, 22260 Pontrieux, France

3Bioalternatives SAS – 1bis rue des plantes – BP N°50011, 86 160 Gençay, France

4SEPPIC – 819 West Nanjing Rd, Room 1508 Zhongchuang Building, Shangai 200041, China

Introduction

Pollution is a global health concern. Skin, being the interface between body and environment, is challenged everyday by atmospheric pollutants (particulate matters , toxic gases, indoor pollutants,…) which contribute to skin disorders such as psoriasis, atopic dermatitis, skin cancer, skin ageing, pigmentary disorders and acne [1–4].

Pollutants are supposed to act through 4 biological mechanisms:

  • generation of free radicals
  • induction of inflammatory cascades
  • activation of aryl hydrocarbon receptor (AhR)lipid
  • alteration of cutaneous microflora [5, 6].

Our previous investigations showed that:

  • Both sebocytes lipid production and skin sebum production are stimulated by some pollutants.
  • Ame* acts as a urban life dermopurifying ingredient, by protecting epidermal cells from cadmium, human sebocytes from urban dust and skin from ozone.

Materials & Methods

lipid production
lipid production

Results & Discussion

1) Lipid droplet formation

lipid production

Pollution: Increase lipid formation.

Ame: Decrease lipid formation (with urban dust)

2) Neutral lipidic neosynthesis

lipid production
lipid production

Conversion of fatty acids into triglycerides:
   Increase with pollution
   Decrease with Ame
lipid production

3) Transcriptomic regulations (24h)

lipid production
lipid production
Only a few regulations observed with pollutants or Ame.
    Putative regulated mechanisms:
         Cyp-pathways
         antioxydant defenses

Conclusion

lipid productionv
Our results:

Enlighten new pollution-induced metabolic regulation of the lipid profile of stimulated sebocytes.

Suggest that underlying mechanisms are more likely to occur at the metabolic level than at the transcriptional level.

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