microRNAs (miRNAs) are small non-coding RNAs (about 22 nucleotides) which play a significant role in the post-transcriptional regulation of genes. miRNAs inhibit the expression of their target genes by binding to the 3’UTR (the three prime untranslated region), thus blocking translation and damage. A mature miRNA can target a lot of genes and conversely, one gene can be the target of several miRNAs.
miRNAs are involved in the regulation of numerous biological functions and are increasingly being used as biomarkers of pathologies, such as cancer, because of their presence in the blood flow and of their high stability (circulating miRNAs).
Many miRNAs have been identified as being involved in the regulation of physiological events in skin and hair, as in the regulation of keratinocyte differentiation or in the ageing mechanism. Some have even been identified as being involved in the development of skin pathologies such as psoriasis.
Bioalternatives can assist you in your project, from miRNA extraction to result interpretation, for miRNome expression by microarrays or only for targeted gene expression by RT-qPCR.