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RNA EXTRACTION AND

QUALITY CONTROL

The RNA extraction from a tissue, from cultured cells or from biological samples is an essential phase for successful transcriptomic analysis and for result interpretation. The good quality of RNA samples relies on the good conditions in which the extraction is performed. This quality will guarantee the later success of your experiments. In order to guarantee optimal results, Bioalternatives offers to pay for the RNA extraction phase of your samples and to perform the RNA quality control.

Types of treated samples

Bioalternatives can perform the RNA extraction step from:

  • Cell culture (monolayers or suspension)
  • Bacteria
  • Reconstructed tissues in culture
  • ex vivo models in culture
  • Tests (blood, tissues)

Recommendations:

When sending samples, it is essential to take the necessary precautions so as to avoid degradation during transport; dry ice packaging is recommended. If sending tissues, preliminary RNAlater treatment is advised.

Extraction step

Bioalternatives can perform the total RNA extraction step of your samples in a standard way.

Bioalternatives can also perform extractions in a more specific way:

  • miRNA
  • mRNA
  • DNA

Applications:

  • RT-qPCR
  • Transcriptome analysis by microarray
  • miRNA expression analysis (RT-qPCR or microarray)

Quality control of RNA

The quality of extracted RNA depends on the conditions in which the samples have been prepared, collected and stored. Once the RNA extraction step is performed, samples require qualitative and quantitative control.

    • The quantity of extracted RNA is determined by spectrophotometry (Nanovue™ spectrophotometer, GE Healthcare)
    • The quality of extracted RNA is measured by capillary electrophoresis, using the Agilent® Bioanalyzer 2100. The level of RNA quality is linked to an INR value determined by the device: the closer this value is to 10, the better the RNA quality is.

CONTACT US

Related Posts or publications

in situ hybridization of nucleic acids in situ hybridization is a technique for visualizing DNA or specific RNA in cells or in a portion of tissue. Its principle is based on the use of specific probes combined with the use of a marker, which may be fluorescent. The probe that is used repr...
Methods of analysis of miRNA expression Bioalternatives offers different solutions for microRNA analysis. The analysis of the whole microRNA expression profile from a DNA/microarray will detect molecular signatures and will identify microRNAs of interest for diagnosis, prognosis or poten...
Gene expression PCR arrays are dedicated to gene expression analysis by quantitative PCR. They are sets of primers selected according to a theme or a given biological process. Gene expression analysis by PCR arrays is considered as the most effective and cost-effici...
Microarrays and transcriptomic analysis Bioalternatives, an Affymetrix® service provider, offers full genome analysis by microarrays (GeneAtlas®platform).
Bioinformatics: basic and functional data processing Bioinformatics services dedicated to transcriptomic data analysis and processing: raw data processing, quality control, normalization, statistical analysis, basic and advanced functional analysis (clusterization and pathway).

SEARCH

Cosmetics

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Health animal and veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)

Online account and catalog access

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Posts and publications

  • New Biological Activities of Lythrum salicaria L.
  • Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
  • Acne vulgaris: Origins, symptoms and current treatments
  • Acne vulgaris: physiopathology and cell mechanisms
  • Physiology and functions of the sebaceous gland

Headquarters

Bioalternatives SAS
1 bis rue des plantes BP 50011
86160 Gençay, France
Tel. +33 (0)5 49 36 11 37
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