A Bioalternatives tem muitos modelos in vitro ou ex vivo à sua disposição:
- Linhagens celulares
- Células imunológicas do sangue periférico (PBMCs e populações purificadas de células sanguínea: subpopulações de linfócitos T, monócitos, células polimorfonucleares etc.)
- Macrófagos e células dendríticas derivadas de monócitos
- Queratinócitos epidérmicos humanos normais (NHEK)
- Fibroblastos dérmicos humanos normais (NHDF)
- Miofibroblastos
- Células tronco mesenquimais
- Células endoteliais microvasculares
- Melanócitos epidérmicos humanos normais (NHEM)
- Neurônios sensoriais
- Epiderme humana reconstruída (RHE)
- Equivalentes dérmicos
- Explantes cutâneos (ex vivo)
nos quais podemos avaliar o efeito pró cicatrização dos compostos ativos, formulações ou dispositivos médicos ao medir:
- resposta inflamatória, limpeza dos ferimentos de pele e imunidade inata
- Re-epitelização
- Neovascularização e neuritogênese
- Síntese de matriz extracelular, fibrose e interação do miofibroblasto
- Remodelamento dérmico e diferenciação epidérmica
- Pigmentação
Dentre todos os ensaios padrão propostos pela Bioalternatives sobre cicatrização de ferimentos e regeneração cutânea, aqui estão alguns exemplos:
Wound healing, granulation phase and maturation phase
/em Wound healing /por GuillaumeThe third phase of wound healing, consisting in the replacement of the provisional fibrin matrix with granulation tissue once the wound has been debrided, includes several sub-phases: re-epithelialization, fibroplasia, collagen deposition and angiogenesis.
Wound healing, hemostasis phase and inflammatory phase
/em Wound healing /por GuillaumeThe inflammatory phase is characterized by the sequential infiltration of polymorphonuclear neutrophils (PMNs), monocytes/macrophages and lymphocytes. IL-8 facilitates PMNs migration from surrounding blood vessels.
Wound healing, overview
/em Wound healing /por GuillaumeWound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells , stem cells , hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves and mechanical forces.
Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children
/em Wound healing /por GuillaumeOur studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested the hypothesis that in vitro studies and RHE reconstructive capacities of cells from different body sites can be helpful to select an optimal site for keratinocyte isolation before considering graft protocols for girls.
Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective
/em Wound healing /por GuillaumeIn the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.
Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts
/em Wound healing /por GuillaumeWe report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. in vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.
Quantitative and qualitative study in keratinocytes from foreskin in children: Perspective application in paediatric burns
/em Wound healing /por GuillaumeKeratinocytes from foreskin have a high capacity for division. A potential source of cells to provide coverage in paediatric burns.
Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study
/em Cell and tissue engineering, Dermatology, Pharmacology, Wound healing /por GuillaumeTo assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells’ capability of constructing a pluristratified epidermis.
Cultured keratinocyte cells from foreskin and future application for burns in children
/em Wound healing /por GuillaumeThe keratinocytes resulting from foreskin have a high capacity of division. These cells can divide a long time before differentiation. The observations enable us to propose with our patients the keratinocytes from foreskin for wound healing especially for burns in children.
Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing
/em Wound healing /por GuillaumeThe effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressing: Mepitel and Tulle Gras.