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Bioalternatives
  • Bem vindos à Bioalternatives
    • Quem somos
    • Nossa equipe
    • Publications
    • Carreiras
  • Cosméticos
    • Hidratação e barreira da pele
    • Regeneração epidérmica
    • Firmeza e coesão da pele
    • Envelhecimento da pele
    • Proteção e defesa da pele
    • Pigmentação da pele
    • Pele oleosa e hiperseborreia
    • Microbioma cutâneo
    • Emagrecimento e metabolismo de adipócito
    • Microcirculação e vascularização da pele
    • Crescimento capilar e alopecia
  • Dermatologia
    • Acne
    • Crescimento capilar e alopecia
    • Cicatrização de ferimentos e regeneração cutânea
    • Dermatite atópica
    • Psoríase
    • Microbioma cutâneo
    • Melanoma
  • Farmacologia in vitro
    • Imuno-inflamação
    • Neurobiologia
    • Saúde animal e medicina veterinária
  • Bioanálise e bioengenharia
    • Engenharia de células e tecidos
    • Quimioinformática, screening virtual in silico & caracterização fitoquímica
      • Screening in silico – SELNERGY
      • Análise fitoquímica & caracterização metabólica – GAINS
    • Expressão de genes e análise do transcriptoma
    • Histologia, imuno-histoquímica, imunofluorescência
      • Coloração histológica
      • Imunomarcação
      • Serviço de microscopia eletrônica – TEM / SEM
  • Menu Menu

CICATRIZACAO

Facilitar a cicatrização cutânea, minimizar a influência estética e restabelecer a função ideal do tecido continuam sendo as preocupações principais dos tratamentos de cicatrização nos setores da indústria dermatológica e cosmética.
A cicatrização de ferimentos é um processo complexo e com várias etapas, baseado no diálogo entre numerosas funções celulares.

O bom conhecimento e a compreensão dos mecanismos celulares e moleculares do processo de cicatrização que participam na regeneração da pele são fundamentais para o desenvolvimento de terapias de tratamento de ferimentos (APIs, biosimilares, formulações, dispositivos médicos).  A Bioalternatives pode oferecer mais de 20 anos de experiência em biologia cutânea em testes in vitro. Aqui se encontram algumas abordagens que oferecemos na área de cicatrização de ferimentos e regeneração da pele para a avaliação de produtos.

Cicatrização de feridas e regeneração da pele: modelos e ensaios in vitro

A Bioalternatives tem muitos modelos in vitro ou ex vivo à sua disposição:

  • Linhagens celulares
  • Células imunológicas do sangue periférico (PBMCs e populações purificadas de células sanguínea: subpopulações de linfócitos T, monócitos, células polimorfonucleares etc.)
  • Macrófagos e células dendríticas derivadas de monócitos
  • Queratinócitos epidérmicos humanos normais (NHEK)
  • Fibroblastos dérmicos humanos normais (NHDF)
  • Miofibroblastos
  • Células tronco mesenquimais
  • Células endoteliais microvasculares
  • Melanócitos epidérmicos humanos normais (NHEM)
  • Neurônios sensoriais
  • Epiderme humana reconstruída (RHE)
  • Equivalentes dérmicos
  • Explantes cutâneos (ex vivo)

nos quais podemos avaliar o efeito pró cicatrização dos compostos ativos, formulações ou dispositivos médicos ao medir:

  • resposta inflamatória, limpeza dos ferimentos de pele e imunidade inata
  • Re-epitelização
  • Neovascularização e neuritogênese
  • Síntese de matriz extracelular, fibrose e interação do miofibroblasto
  • Remodelamento dérmico e diferenciação epidérmica
  • Pigmentação

Dentre todos os ensaios padrão propostos pela Bioalternatives sobre cicatrização de ferimentos e regeneração cutânea, aqui estão alguns exemplos:

  • Pseudotube-formation#Ctrl

    HMVEC + NHDF, differentiation/pseudotube formation (basal, pro-angiogenic agents)

    1.00€
    Comprar Show Details
  • IL-8 release

    NHDF, IL-8 release (IL-1 alpha stimulation)

    1.00€
    Comprar Show Details
  • Dexamethasone

    NHDF, myofibroblast transformation (TGF- beta stimulation)

    1.00€
    Comprar Show Details
  • Procollagen I synthesis

    NHDF, procollagen I synthesis/release (basal)

    1.00€
    Comprar Show Details
  • Cell proliferation

    NHDF, proliferation (basal)

    1.00€
    Comprar Show Details
  • Calcium-induced gene expression

    NHEK, differentiation markers (low calcium/low cell density) (basal)

    1.00€
    Comprar Show Details
  • Cell migration

    NHEK, migration/proliferation (basal)

    1.00€
    Comprar Show Details
  • IL-6-release

    PBMC, LPS-stimulated monocytes, cytokine release (LPS stimulation)

    1.00€
    Comprar Show Details
  • Etiquette

    PMN, migration (basal)

    1.00€
    Comprar Show Details
  • NGF-stimulated

    Sensory neurons, neurite outgrowth (basal)

    1.00€
    Comprar Show Details
AnteriorPróximo
Descubra todos os nossos ensaios padrao - Acesso direto ao catalogo Um projeto, uma idéia ou uma pergunta? Vamos conversar. Entre em contato!

Cicatrização de feridas e regeneração da pele: posts e publications

granulation wound healing

Wound healing, granulation phase and maturation phase

Wound healing

The third phase of wound healing, consisting in the replacement of the provisional  fibrin matrix with granulation tissue once the wound has been debrided, includes several sub-phases: re-epithelialization, fibroplasia, collagen deposition and angiogenesis.

Leia mais
12 novembro 2016/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/11/mw_SMA.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2016-11-12 15:36:082019-09-13 22:08:09Wound healing, granulation phase and maturation phase

Wound healing, hemostasis phase and inflammatory phase

Wound healing

The inflammatory phase is characterized by the sequential infiltration of polymorphonuclear neutrophils (PMNs), monocytes/macrophages and lymphocytes. IL-8 facilitates PMNs migration from surrounding blood vessels.

Leia mais
10 novembro 2016/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/11/mw_blood-cells-cicatrisation-inflammation-AdobeStock_117910248.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2016-11-10 15:40:392019-09-13 22:14:03Wound healing, hemostasis phase and inflammatory phase

Wound healing, overview

Wound healing

Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells , stem cells , hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves and mechanical forces.

Leia mais
9 novembro 2016/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/03/pf_Cicatrisation-Adobe.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2016-11-09 15:45:552019-09-13 22:25:57Wound healing, overview

Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children

Wound healing

Our studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested the hypothesis that in vitro studies and RHE reconstructive capacities of cells from different body sites can be helpful to select an optimal site for keratinocyte isolation before considering graft protocols for girls.

Leia mais
16 setembro 2014/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/03/mw_study-of-proliferation-and-3D-epidermal-reconstruction-from-foreskin.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2014-09-16 16:18:492018-10-24 21:59:38Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children

Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective

Wound healing

In the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.

Leia mais
7 setembro 2014/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/03/mw_epidermal-healing-in-burns.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2014-09-07 16:13:052018-10-24 21:59:50Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Wound healing

We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. in vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.

Leia mais
14 março 2013/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/03/mw_foreskin-isolated-keratinocytes-provide.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2013-03-14 16:32:272018-10-24 22:01:55Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Quantitative and qualitative study in keratinocytes from foreskin in children: Perspective application in paediatric burns

Wound healing

Keratinocytes from foreskin have a high capacity for division. A potential source of cells to provide coverage in paediatric burns.

Leia mais
10 dezembro 2010/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Foreskin-in-children.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2010-12-10 15:11:492018-10-24 22:04:20Quantitative and qualitative study in keratinocytes from foreskin in children: Perspective application in paediatric burns

Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study

Cell and tissue engineering, Dermatology, Pharmacology, Wound healing

To assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells’ capability of constructing a pluristratified epidermis.

Leia mais
21 novembro 2009/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Human-embryonic-stem-cell-derivatives.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2009-11-21 15:43:332019-11-13 11:29:58Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study
burns

Cultured keratinocyte cells from foreskin and future application for burns in children

Wound healing

The keratinocytes resulting from foreskin have a high capacity of division. These cells can divide a long time before differentiation. The observations enable us to propose with our patients the keratinocytes from foreskin for wound healing especially for burns in children.

Leia mais
4 fevereiro 2009/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Cultured-keratinocyte-cells-from-foreskin.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2009-02-04 13:56:522018-10-24 22:05:32Cultured keratinocyte cells from foreskin and future application for burns in children
lipidocolloid dressing

Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing

Wound healing

The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressing: Mepitel and Tulle Gras.

Leia mais
15 maio 2005/por Guillaume
https://www.bioalternatives.com/wp-content/uploads/2016/05/mw_Lipidocolloid-dressing.jpg 368 655 Guillaume https://www.bioalternatives.com/wp-content/uploads/2019/09/Logo-Bioalternatives-150dpisite-web2-300x74.png Guillaume2005-05-15 10:59:162018-10-24 22:14:08Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing

 

 

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250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
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86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Serviço de microscopia eletrônica – TEM / SEM
  • Coloração histológica
  • Imunomarcação

Páginas

  • Acne
  • Bem vindos à Bioalternatives
  • Carreiras
  • Cicatrização de ferimentos e regeneração cutânea
  • Cosméticos
  • Crescimento capilar e alopecia
  • Dermatite atópica
  • Dermatologia
  • Emagrecimento e metabolismo de adipócito
  • Engenharia de células e tecidos
  • Envelhecimento da pele
  • Expressão de genes e análise do transcriptoma
  • Farmacologia in vitro
  • Firmeza e coesão da pele
  • Formulaire – Entre em contato com nossa equipe
  • Hidratação e barreira da pele
  • Histologia, imuno-histoquímica, imunofluorescência
  • Imuno-inflamação
  • Melanoma
  • Microbioma cutâneo
  • Microcirculação e vascularização da pele
  • Neurobiologia
  • Nossa equipe
  • Pele oleosa e hiperseborreia
  • Pigmentação da pele
  • Proteção e defesa da pele
  • Psoríase
  • Quem somos
  • Regeneração epidérmica
  • Saúde animal e medicina veterinária
  • Quimioinformática, screening virtual in silico & caracterização fitoquímica

Categoria

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  • Histology
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