A Bioalternatives tem muitos modelos in vitro à sua disposição:
- Modelo de envelhecimento intrínseco:
- fibroblastos dérmicos humanos “envelhecidos” (modelo de Hayflick)
- equivalente dérmico “envelhecido” (modelo de Hayflick)
- modelo de envelhecimento acelerado:
- fibroblastos dérmicos humanos envelhecidos pelo estresse oxidativo (H2O2)
- pele reconstruída humana em meio deficiente
- modelo de fotoenvelhecimento:
- fibroblastos dérmicos humanos sujeitos à radiação UVA, infravermelha ou UV
- pele reconstruída em espessura total fotoenvelhecida humana
nos quais podemos avaliar o efeito antienvelhecimento dos ingredientes ativos cosméticos sobre:
- renovação celular (proliferação, migração e diferenciação celular)
- síntese e degradação da matriz extracelular (colágeno, elastina, ácido hialurônico, MMPs etc.)
- expressão do marcador de senescência
- produção de radicais livres
Aqui se encontram alguns exemplos dentre todos os ensaios propostos pela Bioalternatives sobre envelhecimento da pele:
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
Cosmetics, Skin ageingDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
Cell and tissue engineering, Skin ageingThe development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
Cosmetics, Cosmétique, Skin ageingThese data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
Cosmetics, Cosmétique, Skin ageingtensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing