A Bioalternatives tem muitos modelos in vitro à sua disposição:
- Modelo de envelhecimento intrínseco:
- fibroblastos dérmicos humanos “envelhecidos” (modelo de Hayflick)
- equivalente dérmico “envelhecido” (modelo de Hayflick)
- modelo de envelhecimento acelerado:
- fibroblastos dérmicos humanos envelhecidos pelo estresse oxidativo (H2O2)
- pele reconstruída humana em meio deficiente
- modelo de fotoenvelhecimento:
- fibroblastos dérmicos humanos sujeitos à radiação UVA, infravermelha ou UV
- pele reconstruída em espessura total fotoenvelhecida humana
nos quais podemos avaliar o efeito antienvelhecimento dos ingredientes ativos cosméticos sobre:
- renovação celular (proliferação, migração e diferenciação celular)
- síntese e degradação da matriz extracelular (colágeno, elastina, ácido hialurônico, MMPs etc.)
- expressão do marcador de senescência
- produção de radicais livres
Aqui se encontram alguns exemplos dentre todos os ensaios propostos pela Bioalternatives sobre envelhecimento da pele:
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
/em Cosmetics, Skin ageing /por GuillaumeDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
/em Cell and tissue engineering, Skin ageing /por GuillaumeThe development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
/em Cosmetics, Cosmétique, Skin ageing /por GuillaumeThese data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
/em Cosmetics, Cosmétique, Skin ageing /por Guillaumetensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing